pWR10

202710 Synthetic Yeast Expression

Plasmid Information

Purpose
episomal bioluminescent reporter plasmid for S. stipitis
Depositor
J. Brian Robertson
Article
Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
Available Since
June 25, 2024
Availability
Available

Sequence Information

Length
7,522 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
CBG99luc — green-emitting variant of click beetle … 2398..4027 Fwd
DNA Sequence
ATGGTGAAGCGTGAGAAAAATGTCATCTATGGCCCTGAGCCTCTCCATCCTTTGGAGGATTTGACTGCCGGCGAAATGTTGTTTCGTGCTCTCCGCAAGCACTCTCATTTGCCTCAAGCCTTGGTCGATGTGGTCGGCGATGAATCTTTGAGCTACAAGGAGTTTTTTGAGGCAACCGTCTTGTTGGCTCAGTCCCTCCACAATTGTGGCTACAAGATGAACGACGTCGTTAGTATCTGTGCTGAAAACAATACCCGTTTCTTCATTCCAGTCATCGCCGCATGGTATATCGGTATGATCGTGGCTCCAGTCAACGAGAGCTACATTCCCGACGAATTGTGTAAAGTCATGGGTATCTCTAAGCCACAGATTGTCTTCACCACTAAGAATATTTTGAACAAAGTCTTGGAAGTCCAAAGCCGCACCAACTTTATTAAGCGTATCATCATCTTGGACACTGTGGAGAATATTCACGGTTGCGAATCTTTGCCTAATTTCATCTCTCGCTATTCAGACGGCAACATCGCAAACTTTAAACCACTCCACTTCGACCCTGTGGAACAAGTTGCAGCCATTTTGTGTAGCAGCGGTACTACTGGACTCCCAAAGGGAGTCATGCAGACCCATCAAAACATTTGCGTGCGTTTGATCCATGCTCTCGATCCACGTGTGGGCACTCAGTTGATTCCTGGTGTCACCGTCTTGGTCTACTTGCCTTTCTTCCATGCTTTCGGCTTTAGCATTACTTTGGGTTACTTTATGGTCGGTCTCCGCGTGATTATGTTCCGCCGTTTTGATCAGGAGGCTTTCTTGAAAGCCATCCAAGATTATGAAGTCCGCAGTGTCATCAACGTGCCTAGCGTGATCTTGTTTTTGTCTAAGAGCCCACTCGTGGACAAGTACGACTTGTCTTCATTGCGTGAATTGTGTTGCGGTGCCGCTCCATTGGCTAAGGAGGTCGCTGAAGTGGCCGCCAAACGCTTGAATCTTCCAGGTATTCGTTGTGGCTTCGGCCTCACCGAATCTACCAGCGCTAACATTCACTCTCTCGGTGATGAGTTTAAGAGCGGCTCTTTGGGCCGTGTCACTCCACTCATGGCTGCTAAGATCGCTGATCGCGAAACTGGTAAGGCTTTGGGTCCGAACCAAGTGGGCGAGTTGTGTATCAAAGGCCCTATGGTGAGCAAGGGTTATGTCAATAACGTTGAAGCTACCAAGGAGGCCATCGACGACGACGGCTGGTTGCATTCTGGTGATTTTGGATATTACGACGAAGATGAGCATTTTTACGTCGTGGATCGTTACAAGGAGTTGATCAAATACAAGGGTAGCCAGGTTGCTCCAGCTGAGTTGGAGGAGATTTTGTTGAAAAATCCATGCATTCGCGATGTCGCTGTGGTCGGCATTCCTGATTTGGAGGCCGGCGAATTGCCTTCTGCTTTCGTTGTCAAGCAGCCTGGTAAAGAAATTACCGCCAAAGAAGTGTATGATTACTTGGCTGAACGTGTGAGCCATACTAAGTACTTGCGTGGCGGCGTGCGTTTTGTTGACTCCATCCCTCGTAACGTAACAGGCAAAATTACCCGCAAGGAGTTGTTGAAACAATTGTTGGAGAAGGCCGGCGGTTAG
Protein Sequence
MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVGDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVNESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLPNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPRVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSVINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVNNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNPCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDSIPRNVTGKITRKELLKQLLEKAGG
AmpR bla beta-lactamase 6525..7386 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 3348..3357 Fwd
DNA Sequence
TAAGGAGGT
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 7386..7491 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 5766..6355 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA