pCOLA-Gent-EM7-Erv1p-DsbC

202485 Escherichia coli Bacterial Expression

Plasmid Information

Purpose
Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively
Depositor
Michael Lynch
Article
Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.
Insert
sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)
Promoter
EM7
Available Since
Nov. 14, 2024
Availability
Available

Sequence Information

Length
3,027 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
EM7 promoter — — 1008..1056 Fwd
DNA Sequence
GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGAC
cat promoter — — 2379..2470 Fwd
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
rep_origin 1
Label Gene Product Position Strand
ColA ori — — 180..816 Rev
DNA Sequence
TGGTGTCGGGAATCCGTAAAGGATCTTCTTGAGATCCTTTTACGATCGTCGTAATCTCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT
terminator 1
Label Gene Product Position Strand
T7Te terminator — — 2346..2374 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG