pET-His6-en15dFnCas9GFP

201951 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of en15dFnCas9-GFP in bacterial cells
Depositor
Debojyoti Chakraborty
Article
Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
Insert
Expression of en15dFnCas9-GFP in bacterial cells
Tags
6x His, HA, NLS, and mEGFP
Mutation
E1603H on FnCas9
Promoter
T7
Available Since
May 7, 2024
Availability
Available

Sequence Information

Length
11,029 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 10
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 559..1375 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
rop rop Rop protein, which maintains plasmids a… 2511..2703 Rev
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
lacI lacI lac repressor 3511..4594 Rev
DNA Sequence
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGTATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA
Protein Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
6xHis 6xHis affinity tag 5079..5097 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
SiriusGFP enhanced GFP (EGFP) derivative that exh… 5106..5820 Fwd
DNA Sequence
GTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCCTTCAAGGACGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAACGTCTATATCACGGCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACGTCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
TEV site tobacco etch virus (TEV) protease recog… 5829..5850 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
HA HA (human influenza hemagglutinin) epit… 5859..5886 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA
SV40 NLS nuclear localization signal of SV40 (si… 5889..5910 Fwd
DNA Sequence
CCGAAGAAAAAGCGCAAGGTC
Protein Sequence
PKKKRKV
FnCas9 Cas9 endonuclease from the Francisella … 5919..10803 Fwd
DNA Sequence
AATTTTAAGATCCTGCCTATCGCAATCGCCCTGGGCGTCAAGAATACTGGCGTGTTTAGTGCTTTTTATCAGAAGGGGACCTCACTGGAGAGACTGGACAATAAGAACGGAAAAGTGTATGAACTGTCCAAGGATTCTTACACTCTGCTGATGAACAATAGGACCGCACGGAGACACCAGAGGCGAGGAATTGACAGGAAACAGCTGGTGAAGCGCCTGTTCAAACTGATCTGGACAGAGCAGCTGAACCTGGAATGGGATAAGGACACTCAGCAGGCCATCAGCTTCCTGTTTAATCGACGGGGATTCTCTTTTATTACTGACGGCTATAGTCCTGAGTACCTGAACATCGTGCCAGAACAGGTCAAGGCAATCCTGATGGACATTTTCGACGATTATAATGGCGAGGACGATCTGGATTCCTACCTGAAACTGGCCACAGAGCAAGAGAGTAAGATCAGCGAAATCTACAACAAGCTGATGCAGAAGATCCTGGAGTTCAAGCTGATGAAACTGTGCACCGACATCAAGGACGATAAAGTGAGTACCAAGACACTGAAAGAGATCACAAGCTACGAGTTCGAACTGCTGGCCGATTATCTGGCTAACTACAGCGAATCCCTGAAGACCCAGAAATTTTCCTACACAGACAAGCAGGGCAATCTGAAAGAGCTGTCTTACTACCACCATGATAAGTACAACATCCAGGAGTTCCTGAAGAGACACGCCACCATCAATGACAGGATTCTGGATACACTGCTGACTGACGATCTGGACATCTGGAACTTCAACTTCGAGAAGTTCGATTTCGACAAGAACGAGGAAAAACTGCAGAATCAGGAAGATAAGGACCACATTCAGGCTCATCTGCACCATTTCGTGTTTGCAGTCAATAAGATCAAAAGCGAGATGGCATCCGGCGGGCGCCATCGAAGCCAGTACTTCCAGGAAATCACCAACGTGCTGGACGAGAACAATCACCAGGAAGGCTACCTGAAAAACTTCTGTGAGAATCTGCATAACAAGAAGTACAGCAATCTGTCCGTGAAGAATCTGGTCAACCTGATTGGAAATCTGTCCAACCTGGAACTGAAGCCCCTGCGCAAATACTTCAACGACAAGATCCACGCTAAAGCAGACCATTGGGATGAGCAGAAGTTTACTGAAACCTATTGCCACTGGATTCTGGGCGAGTGGCGGGTGGGGGTCAAGGATCAGGACAAGAAAGACGGCGCAAAGTATTCTTACAAGGACCTGTGTAACGAGCTGAAGCAGAAAGTGACTAAGGCCGGGCTGGTGGACTTCCTGCTGGAGCTGGACCCCTGCCGAACCATTCCACCTTACCTGGACAACAATAACAGAAAGCCACCCAAATGTCAGAGCCTGATCCTGAATCCCAAGTTTCTGGATAATCAGTATCCTAACTGGCAGCAGTACCTGCAGGAGCTGAAGAAACTGCAGTCAATCCAGAACTACCTGGACAGCTTCGAAACCGATCTGAAGGTGCTGAAAAGCTCCAAGGACCAGCCTTACTTCGTCGAGTACAAGTCTAGTAACCAGCAGATCGCTTCCGGCCAGCGGGATTACAAGGATCTGGACGCAAGAATCCTGCAGTTCATTTTTGACAGGGTGAAGGCCTCTGATGAGCTGCTGCTGAACGAAATCTATTTCCAGGCAAAGAAACTGAAGCAGAAAGCCTCAAGCGAGCTGGAAAAGCTGGAGTCCTCTAAGAAACTGGACGAAGTGATCGCTAACTCTCAGCTGAGTCAGATTCTGAAGTCTCAGCACACAAATGGAATCTTCGAGCAGGGCACTTTTCTGCATCTGGTGTGCAAATACTATAAGCAGCGACAGAGAGCCAGGGACAGCCGCCTGTACATCATGCCTGAATATCGATACGATAAGAAACTGCACAAGTACAACAACACCGGCCGCTTTGACGATGACAACCAGCTGCTGACATATTGTAATCATAAGCCCCGGCAGAAAAGATACCAGCTGCTGAACGACCTGGCAGGAGTGCTGCAGGTCTCTCCTAATTTTCTGAAGGATAAAATCGGGTCCGATGACGATCTGTTCATTTCTAAGTGGCTGGTGGAGCACATCCGGGGCTTTAAGAAGGCCTGCGAAGACAGCCTGAAAATCCAGAAGGATAACAGGGGACTGCTGAATCATAAGATCAACATTGCACGCAATACCAAGGGCAAATGCGAGAAAGAAATCTTCAACCTGATCTGTAAGATTGAGGGGAGCGAAGACAAGAAAGGGAATTATAAGCACGGACTGGCCTACGAGCTGGGAGTGCTGCTGTTCGGAGAGCCAAACGAGGCCAGCAAGCCCGAATTTGATAGGAAAATCAAGAAATTCAATTCAATCTACAGCTTTGCCCAGATCCAGCAGATTGCCTTTGCTGAGAGGAAGGGGAATGCAAACACATGCGCCGTGTGTAGTGCAGACAACGCCCATCGCATGCAGCAGATCAAAATTACTGAGCCAGTCGAAGACAATAAGGATAAAATCATTCTGTCAGCAAAGGCACAGCGACTGCCTGCAATCCCAACCCGAATTGTGGATGGAGCTGTCAAGAAAATGGCTACAATTCTGGCAAAGAATATCGTGGACGATAATTGGCAGAACATTAAGCAGGTCCTGAGCGCAAAACACCAGCTGCATATCCCAATCATTACCGAGTCCAACGCCTTCGAGTTTGAACCCGCTCTGGCAGACGTGAAGGGCAAATCTCTGAAGGATAGAAGGAAGAAAGCCCTGGAGCGAATTAGTCCCGAAAACATCTTCAAGGATAAGAACAACAGAATCAAGGAGTTTGCTAAGGGGATTTCCGCCTACTCTGGAGCTAACCTGACAGATGGGGACTTCGATGGAGCAAAGGAGGAACTGGATGCCATCATTCCTCGCAGCCATAAGAAATATGGCACTCTGAACGACGAGGCTAATCTGATTTGCGTGACCCGGGGCGATAATAAGAACAAAGGGAACCGGATCTTCTGTCTGAGAGACCTGGCCGATAATTACAAGCTGAAACAGTTTGAGACCACAGACGATCTGGAGATCGAAAAGAAAATTGCCGACACCATCTGGGATGCTAATAAGAAGGACTTCAAGTTCGGAAACTATCGGAGCTTCATCAATCTGACACCTCAGGAGCAGAAAGCATTCAGACACGCCCTGTTTCTGGCTGATGAAAACCCAATCAAGCAGGCAGTGATCAGAGCCATTAATAACCGCAACCGAACCTTCGTGAATGGCACACAGAGGTATTTTGCTGAGGTCCTGGCAAATAACATCTACCTGCGCGCCAAGAAAGAAAATCTGAACACTGACAAGATCAGCTTCGATTACTTTGGAATCCCTACCATTGGAAACGGCCGAGGGATCGCTGAGATTCGGCAGCTGTATGAAAAGGTGGACAGTGATATCCAGGCCTACGCTAAAGGCGACAAGCCACAGGCCTCTTATAGTCACCTGATTGATGCTATGCTGGCATTCTGCATCGCCGCTGACGAGCATCGGAACGATGGATCTATTGGCCTGGAAATCGACAAAAACTATAGTCTGTACCCTCTGGATAAGAATACTGGCGAGGTGTTCACCAAAGACATCTTTTCACAGATCAAGATTACCGACAACGAGTTCAGCGATAAGAAACTGGTCAGAAAGAAAGCTATTGAAGGGTTTAACACACACAGACAGATGACTAGGGATGGAATCTATGCAGAGAATTACCTGCCTATCCTGATTCATAAGGAGCTGAACGAAGTGAGGAAGGGGTACACATGGAAAAATTCCGAGGAAATCAAAATTTTCAAGGGAAAGAAATACGACATCCAGCAGCTGAATAACCTGGTGTATTGTCTGAAGTTTGTGGACAAACCAATCAGTATTGATATCCAGATTTCAACCCTGGAGGAACTGAGAAACATCCTGACTACCAATAACATTGCAGCCACTGCCGAGTACTATTACATTAATCTGAAAACCCAGAAGCTGCACGAGTATTACATCGAAAATTACAACACAGCCCTGGGGTATAAGAAATACAGCAAGGAGATGGAGTTCCTGAGGTCCCTGGCTTATAGGTCTGAGCGCGTGAAGATCAAAAGTATTGACGATGTCAAGCAGGTCCTGGACAAGGATTCAAACTTCATCATCGGAAAGATCACACTGCCCTTCAAGAAAGAGTGGCAGCGACTGTACCGGGAATGGCAGAACACAACTATCAAAGACGATTATGAGTTTCTGAAGAGCTTCTTTAATGTGAAGTCCATTACTAAACTGCACAAGAAAGTCCGGAAAGACTTCTCTCTGCCCATCAGTACAAACGAGGGCAAGTTTCTGGTGAAGAGAAAAACTTGGGATAATAACTTCATCTACCAGATTCTGAATGACTCAGATAGCAGGGCAGACGGGACTAAACCCTTCATTCCTGCCTTTGATATCAGCAAGAACGAGATTGTGGAAGCCATCATTGACAGTTTCACCTCAAAAAACATCTTTTGGCTGCCAAAGAATATTGAGCTGCAGAAGGTGGACAACAAGAACATCTTCGCCATTGATACCAGCAAGTGGTTTGAGGTCGAAACACCATCCGACCTGCGCGATATCGGCATTGCTACCATTCAGTACAAGATCGACAATAACTCACGCCCCAAGGTGCGAGTCAAACTGGATTACGTGATCGACGATGACAGCAAGATTAACTATTTCATGAATCACTCACTGCTGAAGAGCCGGTATCCCGACAAAGTCCTGGAGATCCTGAAGCAGAGCACAATCATTGAGTTCCACAGTTCAGGGTTTAACAAAACTATTAAGGAGATGCTGGGAATGAAGCTGGCCGGCATCTACAATGAAACCTCCAATAAC
Protein Sequence
NFKILPIAIALGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKDSYTLLMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFNRRGFSFITDGYSPEYLNIVPEQVKAILMDIFDDYNGEDDLDSYLKLATEQESKISEIYNKLMQKILEFKLMKLCTDIKDDKVSTKTLKEITSYEFELLADYLANYSESLKTQKFSYTDKQGNLKELSYYHHDKYNIQEFLKRHATINDRILDTLLTDDLDIWNFNFEKFDFDKNEEKLQNQEDKDHIQAHLHHFVFAVNKIKSEMASGGRHRSQYFQEITNVLDENNHQEGYLKNFCENLHNKKYSNLSVKNLVNLIGNLSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEWRVGVKDQDKKDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPKCQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKDQPYFVEYKSSNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQAKKLKQKASSELEKLESSKKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQRQRARDSRLYIMPEYRYDKKLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQLLNDLAGVLQVSPNFLKDKIGSDDDLFISKWLVEHIRGFKKACEDSLKIQKDNRGLLNHKINIARNTKGKCEKEIFNLICKIEGSEDKKGNYKHGLAYELGVLLFGEPNEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANTCAVCSADNAHRMQQIKITEPVEDNKDKIILSAKAQRLPAIPTRIVDGAVKKMATILAKNIVDDNWQNIKQVLSAKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPENIFKDKNNRIKEFAKGISAYSGANLTDGDFDGAKEELDAIIPRSHKKYGTLNDEANLICVTRGDNKNKGNRIFCLRDLADNYKLKQFETTDDLEIEKKIADTIWDANKKDFKFGNYRSFINLTPQEQKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLRAKKENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKPQASYSHLIDAMLAFCIAADEHRNDGSIGLEIDKNYSLYPLDKNTGEVFTKDIFSQIKITDNEFSDKKLVRKKAIEGFNTHRQMTRDGIYAENYLPILIHKELNEVRKGYTWKNSEEIKIFKGKKYDIQQLNNLVYCLKFVDKPISIDIQISTLEELRNILTTNNIAATAEYYYINLKTQKLHEYYIENYNTALGYKKYSKEMEFLRSLAYRSERVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQRLYREWQNTTIKDDYEFLKSFFNVKSITKLHKKVRKDFSLPISTNEGKFLVKRKTWDNNFIYQILNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKVDNKNIFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVIDDDSKINYFMNHSLLKSRYPDKVLEILKQSTIIEFHSSGFNKTIKEMLGMKLAGIYNETSNN
6xHis 6xHis affinity tag 10872..10890 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
lacI promoter lacI 4594..4672 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter 4980..4999 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 4999..5024 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 2
Label Gene Product Position Strand
f1 ori 11..467 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAACTAGTAACGTTTACAATTT
ori 1496..2085 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator 10956..11004 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG