pMflT-o4-mNG

201855 Synthetic Bacterial Expression

Plasmid Information

Purpose
Mesoplasma florum oriC plasmid expressing mNeonGreen fluorescent protein
Depositor
Sébastien Rodrigue
Insert
mNeonGreen
Available Since
Oct. 10, 2023
Availability
Available

Sequence Information

Length
6,271 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

promoter 3
Label Gene Product Position Strand
T7 promoter — — 3956..3975 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T7 promoter — — 4875..4894 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T3 promoter — — 5595..5614 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
rep_origin 1
Label Gene Product Position Strand
ori — — 5001..5590 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACAACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA