AAVTRE3_smFP_HA

201209 Synthetic Bacterial Expression

Plasmid Information

Purpose
AAV vector for TRE(TRE3G)-driven smFP_HA (HA-tagged spaghetti monster) expression
Depositor
Tetsuo Yamamori
Article
Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37
Insert
smFP _HA (addgene plasmid #59759)
Promoter
TRE3G
Available Since
Aug. 14, 2025
Availability
Available

Sequence Information

Length
5,523 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 12
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 556..583 Fwd
DNA Sequence
TACCCTTATGATGTGCCCGATTATGCT
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 586..613 Fwd
DNA Sequence
TACCCTTATGATGTTCCTGATTACGCC
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 616..643 Fwd
DNA Sequence
TATCCGTATGACGTGCCAGACTACGCG
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1171..1198 Fwd
DNA Sequence
TATCCCTACGACGTGCCCGATTATGCG
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1204..1231 Fwd
DNA Sequence
TATCCGTATGATGTTCCAGATTATGCT
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1243..1270 Fwd
DNA Sequence
TATCCCTATGATGTCCCCGACTACGCC
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1276..1303 Fwd
DNA Sequence
TACCCATACGACGTGCCCGACTACGCT
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1513..1540 Fwd
DNA Sequence
TACCCCTACGATGTGCCGGATTACGCT
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1543..1570 Fwd
DNA Sequence
TATCCGTATGACGTACCGGACTATGCC
Protein Sequence
YPYDVPDYA
HA — HA (human influenza hemagglutinin) epit… 1573..1600 Fwd
DNA Sequence
TACCCGTACGATGTCCCGGACTACGCT
Protein Sequence
YPYDVPDYA
Factor Xa site — Factor Xa recognition and cleavage site 2120..2132 Rev
DNA Sequence
ATTGAGGGCCGA
Protein Sequence
IEGR
AmpR bla beta-lactamase 3842..4703 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
misc_feature 1
Label Gene Product Position Strand
WPRE — — 1649..2238 Fwd
DNA Sequence
AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC
polyA_signal 1
Label Gene Product Position Strand
hGH poly(A) signal — — 2269..2746 Fwd
DNA Sequence
GGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT
promoter 2
Label Gene Product Position Strand
TRE3G promoter — — 146..525 Fwd
DNA Sequence
GAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATAAGCTTTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACCAACTTTCCGTACCACTTCCTACCCTCGTAAA
AmpR promoter bla — 3737..3842 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 7
Label Gene Product Position Strand
tet operator tetO — 154..173 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 190..209 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 226..245 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 262..281 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 298..317 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 334..353 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 370..389 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 3000..3456 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT
ori — — 4873..5462 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA