AAVTRE3_smFP_Myc

201208 Synthetic Bacterial Expression

Plasmid Information

Purpose
AAV vector for TRE(TRE3G)-driven smFP_Myc (Myc-tagged spaghetti monster) expression
Depositor
Tetsuo Yamamori
Article
Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37
Insert
smFP _Myc (from addgene #59757)
Promoter
TRE3G
Available Since
Aug. 14, 2025
Availability
Available

Sequence Information

Length
5,559 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 12
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 556..586 Fwd
DNA Sequence
GAGCAAAAACTGATATCGGAGGAAGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 589..619 Fwd
DNA Sequence
GAGCAGAAACTGATTTCGGAAGAGGATCTT
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 622..652 Fwd
DNA Sequence
GAACAAAAACTGATCAGTGAGGAAGACCTT
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1183..1213 Fwd
DNA Sequence
GAACAGAAGCTCATCAGTGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1219..1249 Fwd
DNA Sequence
GAGCAGAAACTCATTAGCGAGGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1261..1291 Fwd
DNA Sequence
GAGCAGAAGCTGATATCGGAGGAAGACCTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1297..1327 Fwd
DNA Sequence
GAGCAAAAGCTCATCTCGGAGGAGGATCTC
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1540..1570 Fwd
DNA Sequence
GAACAGAAACTTATCTCCGAGGAGGATCTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1573..1603 Fwd
DNA Sequence
GAACAGAAGCTGATCAGCGAAGAGGACCTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 1606..1636 Fwd
DNA Sequence
GAGCAGAAACTTATCAGCGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
Factor Xa site — Factor Xa recognition and cleavage site 2156..2168 Rev
DNA Sequence
ATTGAGGGCCGA
Protein Sequence
IEGR
AmpR bla beta-lactamase 3878..4739 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
misc_feature 1
Label Gene Product Position Strand
WPRE — — 1685..2274 Fwd
DNA Sequence
AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC
polyA_signal 1
Label Gene Product Position Strand
hGH poly(A) signal — — 2305..2782 Fwd
DNA Sequence
GGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT
promoter 2
Label Gene Product Position Strand
TRE3G promoter — — 146..525 Fwd
DNA Sequence
GAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATAAGCTTTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACCAACTTTCCGTACCACTTCCTACCCTCGTAAA
AmpR promoter bla — 3773..3878 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 7
Label Gene Product Position Strand
tet operator tetO — 154..173 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 190..209 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 226..245 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 262..281 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 298..317 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 334..353 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 370..389 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 3036..3492 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT
ori — — 4909..5498 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA