Atg20-24 pST39

200439 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.
Depositor
Michael Ragusa
Article
Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/
Tags
6xHis-TEV
Available Since
July 12, 2023
Availability
Available

Sequence Information

Length
6,093 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
rop rop Rop protein, which maintains plasmids a… 1015..1207 Rev
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
6xHis — 6xHis affinity tag 2874..2892 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 2892..2913 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
promoter 2
Label Gene Product Position Strand
T7 promoter — — 1443..1462 Fwd
DNA Sequence
TAATACGACTCACTATAGG
AmpR promoter bla — 5067..5159 Fwd
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 0..589 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
T7 terminator — — 4953..5001 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
fd terminator — — 6023..6063 Fwd
DNA Sequence
GATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGA