pLP-pR-himar-apraR

199996 Synthetic Bacterial Expression

Plasmid Information

Purpose
Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor
Depositor
Farren Isaacs
Article
Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.
Insert
Landing Pads
Available Since
Nov. 9, 2023
Availability
Available

Sequence Information

Length
9,919 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
superfolder GFP — GFP variant that folds robustly even wh… 4859..5573 Fwd
DNA Sequence
ATGAGCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAGGCGATGCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCCGGTTCCGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGTTACCCGGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATGTCCAAGAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGTGAAATTCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAGGAAGACGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGTACATCACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAATGTGGAAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGTGATGGCCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTAAAGATCCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGGCATCACGCACGGTATGGACGAACTGTATAAA
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
promoter 3
Label Gene Product Position Strand
T7 promoter — — 4813..4832 Fwd
DNA Sequence
TAATACGACTCACTATAGG
SP6 promoter — — 6180..6199 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
AmpR promoter bla — 6255..6347 Fwd
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori — — 9516..9905 Fwd
DNA Sequence
GATCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCCTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACA
terminator 1
Label Gene Product Position Strand
fd terminator — — 7211..7251 Fwd
DNA Sequence
GATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGA