pLP-pTac-himar-apraR

199995 Synthetic Bacterial Expression

Plasmid Information

Purpose
Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.
Depositor
Farren Isaacs
Article
Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.
Insert
Landing Pads
Mutation
himar frameshift
Available Since
March 14, 2024
Availability
Available

Sequence Information

Length
8,921 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
superfolder GFP — GFP variant that folds robustly even wh… 4582..5296 Fwd
DNA Sequence
ATGAGCAAGGGCGAAGAACTGTTTACGGGCGTGGTGCCGATTCTGGTGGAACTGGATGGTGATGTCAATGGTCACAAATTCAGCGTGCGCGGCGAAGGTGAAGGCGATGCAACCAATGGTAAACTGACGCTGAAGTTTATTTGCACCACGGGTAAACTGCCGGTTCCGTGGCCGACCCTGGTCACCACGCTGACGTATGGTGTTCAGTGTTTCAGTCGTTACCCGGATCACATGAAACGCCACGACTTTTTCAAGTCCGCGATGCCGGAAGGTTATGTCCAAGAACGTACCATCTCATTTAAAGATGACGGCACCTACAAAACGCGCGCCGAAGTGAAATTCGAAGGTGATACGCTGGTTAACCGTATTGAACTGAAAGGCATCGATTTTAAGGAAGACGGTAATATTCTGGGCCATAAACTGGAATATAACTTCAATTCGCACAACGTGTACATCACCGCAGATAAGCAGAAGAACGGTATCAAGGCTAACTTCAAGATCCGCCATAATGTGGAAGATGGCAGCGTTCAACTGGCCGACCACTATCAGCAAAACACCCCGATTGGTGATGGCCCGGTCCTGCTGCCGGACAATCATTACCTGAGCACGCAGTCTGTGCTGAGTAAAGATCCGAACGAAAAGCGTGACCACATGGTCCTGCTGGAATTCGTGACCGCGGCCGGCATCACGCACGGTATGGACGAACTGTATAAA
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
AmpR bla beta-lactamase 7341..8202 Fwd
DNA Sequence
ATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCTTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCAAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGTCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 4
Label Gene Product Position Strand
T7 promoter — — 4536..4555 Fwd
DNA Sequence
TAATACGACTCACTATAGG
tac promoter — — 5923..5952 Fwd
DNA Sequence
TTGACAATTAATCATCGGCTCGTATAATG
SP6 promoter — — 7113..7132 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
AmpR promoter bla — 7249..7341 Fwd
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTAATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 5955..5979 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTC
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori — — 8518..8907 Fwd
DNA Sequence
GATCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCCTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACA
terminator 1
Label Gene Product Position Strand
fd terminator — — 8205..8245 Fwd
DNA Sequence
GATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGA