pARSCL212

198638 Synthetic Bacterial Expression

Plasmid Information

Purpose
Level 0 35S:Ω:Ruby:t35S component
Depositor
Shaun Curtin
Insert
35S:Ω:Ruby:t35S
Available Since
May 18, 2023
Availability
Available

Sequence Information

Length
7,620 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
RUBY — — 1587..5538 Fwd
DNA Sequence
ATGGATCATGCGACCCTCGCCATGATCCTCGCGATCTGGTTCATCAGCTTCCACTTCATCAAGCTGCTGTTCTCCCAGCAGACCACCAAGCTGCTTCCGCCAGGACCAAAGCCGCTTCCGATCATCGGCAACATCCTTGAGGTGGGCAAGAAGCCGCATCGGTCCTTCGCCAACCTCGCCAAGATTCACGGCCCACTCATTTCCCTCAGACTCGGCTCTGTGACCACCATCGTTGTGTCCTCTGCCGACGTGGCCAAAGAGATGTTCCTCAAGAAGGATCACCCGCTCTCCAACCGCACGATCCCGAATAGTGTTACAGCCGGCGACCACCACAAGCTCACCATGTCTTGGCTCCCGGTGTCTCCGAAGTGGCGCAACTTCCGCAAGATTACCGCCGTGCATCTGCTCTCCCCACAGAGACTCGATGCCTGCCAGACATTCAGGCACGCCAAGGTGCAGCAGCTCTACGAGTACGTTCAAGAGTGCGCCCAGAAAGGCCAGGCCGTGGATATTGGCAAGGCCGCCTTTACGACCAGCCTCAACCTCCTCAGCAAGCTGTTCTTCAGCGTCGAGCTGGCGCACCACAAGTCCCATACCAGCCAAGAGTTCAAAGAGCTGATCTGGAACATCATGGAAGATATAGGCAAGCCGAACTACGCCGACTACTTCCCGATTCTCGGCTGCGTTGACCCATCTGGCATTAGAAGAAGGCTCGCCTGCTCCTTCGACAAGCTGATCGCCGTGTTCCAGGGCATCATCTGCGAGAGACTCGCCCCAGATTCCTCCACCACAACTACCACCACCACCGACGACGTGCTCGATGTGCTCCTCCAGCTGTTCAAGCAGAACGAGCTGACGATGGGCGAGATCAACCACCTCCTCGTGGACATCTTCGACGCCGGCACCGATACCACATCCTCCACATTCGAGTGGGTGATGACCGAGCTGATCCGCAATCCAGAGATGATGGAAAAGGCCCAAGAGGAAATCAAGCAGGTCCTCGGCAAGGACAAGCAGATCCAAGAGTCCGACATCATCAACCTGCCGTACCTCCAGGCGATCATCAAAGAGACACTCCGCCTCCATCCGCCGACCGTGTTCTTGCTCCCAAGAAAGGCCGACACCGATGTCGAGCTGTACGGCTACATCGTGCCGAAGGATGCCCAGATCCTCGTGAACCTCTGGGCCATTGGCAGGGACCCAAACGCCTGGCAGAACGCCGATATTTTCAGCCCAGAGCGCTTCATCGGCTGCGAGATCGATGTTAAGGGCCGCGATTTCGGCCTCCTTCCATTTGGCGCTGGCCGCAGAATTTGCCCAGGCATGAATCTCGCCATCAGGATGCTCACCCTCATGCTCGCCACACTCCTCCAGTTCTTCAACTGGAAGCTCGAAGGCGACATCTCCCCGAAGGACCTCGACATGGACGAGAAGTTCGGCATTGCGCTCCAAAAGACCAAGCCGCTCAAGCTCATCCCGATTCCGCGCTACGGTAGCGGAGCTACCAATTTTAGCCTCCTTAAGCAGGCTGGTGATGTAGAAGAGAACCCCGGGCCTATGAAGATGATGAACGGCGAGGACGCCAACGACCAGATGATCAAAGAGTCCTTCTTCATCACCCACGGCAACCCGATCCTCACCGTCGAGGATACACATCCGCTCAGGCCGTTCTTCGAGACATGGCGCGAGAAGATTTTCTCCAAGAAGCCGAAGGCCATCCTCATCATCTCCGGCCACTGGGAGACAGTGAAGCCAACCGTGAACGCCGTGCACATCAACGACACCATCCACGACTTCGACGACTACCCAGCCGCCATGTACCAGTTCAAGTACCCAGCTCCAGGCGAGCCAGAGCTTGCGAGAAAGGTGGAAGAGATCCTCAAGAAGTCCGGGTTCGAGACAGCCGAGACAGACCAAAAGAGGGGCCTTGATCACGGCGCCTGGGTTCCACTCATGCTCATGTATCCAGAGGCGGACATCCCGGTGTGCCAGCTCTCAGTTCAGCCACATCTCGACGGCACCTACCACTACAATCTCGGCAGAGCCCTCGCGCCGCTCAAGAATGATGGCGTGCTCATTATTGGCTCCGGCAGCGCCACACATCCACTCGATGAGACACCGCACTACTTCGATGGTGTTGCCCCTTGGGCCGCTGCCTTCGATTCTTGGCTTAGGAAGGCCCTCATCAACGGCCGCTTCGAGGAAGTGAACATCTACGAGAGCAAGGCCCCGAACTGGAAGCTCGCCCATCCATTTCCAGAGCACTTCTACCCGCTCCACGTTGTGCTCGGCGCTGCTGGTGAAAAGTGGAAGGCCGAGCTGATCCACTCCTCCTGGGATCATGGCACACTTTGCCACGGCTCCTACAAGTTCACCTCCGCCGGATCCGGAGCAACCAACTTTAGCCTGCTCAAGCAAGCAGGAGATGTTGAGGAAAATCCTGGCCCCATGACCGCCATCAAGATGAACACCAACGGCGAGGGCGAGACACAGCACATCCTCATGATCCCGTTCATGGCGCAGGGCCACCTCAGGCCATTTCTCGAACTCGCCATGTTCCTCTACAAGCGCTCCCACGTGATCATCACCCTGCTCACAACTCCGCTCAATGCCGGCTTCCTCAGGCACCTCCTTCACCACCATTCCTACTCCTCCAGCGGCATCAGGATCGTCGAGCTGCCATTCAACTCCACCAACCACGGACTCCCACCGGGCATCGAGAACACCGATAAGCTCACACTCCCGCTCGTGGTGTCCCTCTTCCATTCCACCATCAGCCTCGATCCGCACCTCCGCGATTACATCTCCAGGCATTTCAGCCCAGCCAGGCCACCACTCTGCGTGATCCATGATGTGTTCCTCGGCTGGGTTGACCAGGTGGCCAAGGATGTGGGCTCTACAGGCGTGGTGTTCACAACAGGCGGCGCTTATGGCACATCCGCCTACGTGTCCATCTGGAACGATCTCCCGCACCAGAACTACTCCGACGACCAAGAGTTCCCGCTGCCAGGCTTCCCAGAGAACCATAAGTTCCGCAGGTCCCAGCTCCATCGGTTCCTCAGATATGCCGACGGCTCCGACGATTGGTCCAAGTATTTCCAGCCGCAGCTCCGCCAGTCCATGAAGTCTTTTGGCTGGCTCTGCAACTCCGTGGAAGAGATCGAGACACTCGGCTTCTCCATCCTCCGCAACTACACCAAGCTGCCGATCTGGGGCATCGGCCCACTTATTGCTTCCCCAGTGCAGCACTCCTCCTCCGACAACAATTCAACAGGCGCCGAGTTCGTGCAGTGGCTCAGCCTCAAAGAGCCGGACTCCGTCCTCTACATCTCCTTCGGCTCCCAGAACACGATCAGCCCGACGCAGATGATGGAACTCGCTGCTGGCCTTGAGTCCTCCGAGAAGCCATTCCTCTGGGTGATCAGAGCCCCGTTCGGCTTCGACATCAACGAAGAGATGCGCCCAGAGTGGCTGCCAGAGGGCTTTGAGGAACGCATGAAGGTGAAGAAACAGGGCAAGCTCGTGTACAAGCTCGGCCCGCAGCTTGAGATCCTCAACCATGAATCCATCGGCGGCTTTCTCACCCACTGCGGATGGAACAGCATCCTTGAGTCTCTTCGCGAGGGCGTTCCGATGCTTGGATGGCCACTTGCTGCCGAGCAGGCCTACAACCTCAAGTACCTCGAAGATGAGATGGGCGTCGCGGTTGAGCTTGCTAGAGGCCTCGAAGGCGAGATCTCCAAAGAGAAGGTCAAGCGCATCGTCGAGATGATCCTTGAGCGCAACGAGGGCTCCAAAGGCTGGGAGATGAAGAATCGCGCCGTGGAAATGGGCAAAAAGCTCAAGGACGCCGTGAACGAGGAAAAAGAGCTGAAGGGCTCCTCCGTGAAGGCGATCGACGATTTCCTCGACGCCGTCATGCAGGCCAAACTTGAGCCAAGCCTCCAGTGA
P2A — 2A peptide from porcine teschovirus-1 p… 3087..3144 Fwd
DNA Sequence
GCTACCAATTTTAGCCTCCTTAAGCAGGCTGGTGATGTAGAAGAGAACCCCGGGCCT
Protein Sequence
ATNFSLLKQAGDVEENPGP
P2A — 2A peptide from porcine teschovirus-1 p… 3978..4035 Fwd
DNA Sequence
GCAACCAACTTTAGCCTGCTCAAGCAAGCAGGAGATGTTGAGGAAAATCCTGGCCCC
Protein Sequence
ATNFSLLKQAGDVEENPGP
polyA_signal 1
Label Gene Product Position Strand
CaMV poly(A) signal — — 5578..5753 Fwd
DNA Sequence
TTTCTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTACTAAAATCCAGATC
promoter 2
Label Gene Product Position Strand
CaMV 35S promoter — — 1166..1512 Fwd
DNA Sequence
TGAGACTTTTCAACAAAGGATAATTTCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCGAAAGGACAGTAGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCATTCAAGATCTCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGAGGTTCCAACCACGTCTACAAAGCAAGTGGATTGATGTGACATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
AmpR promoter bla — 5822..5927 Fwd
DNA Sequence
CGATGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTAATGAGACAATAACCCTGATAAATGCTTCAATTATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 6994..7583 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACTAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCTCACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCTGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA