pET19b NFRKB 1-101

19787 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joan Conaway, Ronald Conaway
Article
Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.
Insert
NFRKB (NFRKB Human)
Tags
His
Mutation
only contains amino acids 1-101
Available Since
Nov. 21, 2008
Availability
Available

Sequence Information

Length
808 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
enterokinase site — enterokinase recognition and cleavage s… 360..375 Rev
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
10xHis — 10xHis affinity tag 390..420 Rev
DNA Sequence
CATCATCATCATCATCATCATCATCATCAC
Protein Sequence
HHHHHHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 494..513 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 469..494 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC