pET3a-Ub-K48C

18897 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cecile Pickart
Article
Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1
Insert
Ubiquitin-K48C (UBC Human)
Mutation
Lysine 48 is replaced with a Cysteine residue
Available Since
Sept. 10, 2008
Availability
Available

Sequence Information

Length
852 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 44..272 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAACCATCCGATACCATCGAAAACGTTAAGGCTAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTTGCCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGCQLEDGRTLSDYNIQKESTLHLVLRLRGG
terminator 1
Label Gene Product Position Strand
T7 terminator — — 339..387 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG