pET3a-Ub-D77

18896 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cecile Pickart
Article
Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1
Insert
Ubiquitin-D77 (UBC Human)
Mutation
Aspartate residue is added to the C-terminus of t…
Available Since
Sept. 10, 2008
Availability
Available

Sequence Information

Length
859 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 45..273 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAACCATCCGATACCATCGAAAACGTTAAGGCTAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
promoter 1
Label Gene Product Position Strand
tet promoter 756..785 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator 343..391 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG