pTXTL-T7max-LuxC

188307 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of LuxC via a T7 promoter in TXTL
Depositor
Kate Adamala
Article
Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10
Insert
luxC
Promoter
T7Max
Available Since
Oct. 17, 2022
Availability
Available

Sequence Information

Length
4,316 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
LuxC luxC LuxC fatty acid reductase 103..1546 Fwd
DNA Sequence
ATGACTAAAAAAATTTCATTCATTATTAACGGCCAGGTTGAAATCTTTCCCGAAGGTGATGATTTAGTGCAATCCATTAATTTTGGTGATAATAGTGTTTACCTGCCAATATTGAATGACTCTCATGTAAAAAACATTATTGATTGTAATGGAAATAACGAATTACGGTTGCATAACATTGTCAATTTTCTCTATACGGTAGGGCAAAGATGGAAAAATGAAGAATACTCAAGACGCAGGACATACATTCGTGACTTAAAAAAATATATGGGATATTCAGAAGAAATGGCTAAGCTAGAGGCCAATTGGATATCTATGATTTTATGTTCTAAAGGCGGCCTTTATGATGTTGTAGAAAATGAACTTGGTTCTCGCCATATCATGGATGAATGGCTACCTCAGGATGAAAGTTATGTTCGGGCTTTTCCGAAAGGTAAATCTGTACATCTGTTGGCAGGTAATGTTCCATTATCTGGGATCATGTCTATATTACGCGCAATTTTAACTAAGAATCAGTGTATTATAAAAACATCGTCAACCGATCCTTTTACCGCTAATGCATTAGCGTTAAGTTTTATTGATGTAGACCCTAATCATCCGATAACGCGCTCTTTATCTGTTATATATTGGCCCCACCAAGGTGATACATCACTCGCAAAAGAAATTATGCAACATGCGGATGTTATTGTCGCTTGGGGAGGGCCAGATGCGATTAATTGGGCGGTAGAGCATGCGCCATCTTATGCTGATGTGATTAAATTTGGTTCTAAAAAGAGTCTTTGCATTATCGATAATCCTGTTGATTTGACGTCCGCAGCGACAGGTGCGGCTCATGATGTTTGTTTTTACGATCAGCGAGCTTGTTTTTCTGCCCAAAACATATATTACATGGGAAATCATTATGAGGAATTTAAGTTAGCGTTGATAGAAAAACTTAATCTATATGCGCATATATTACCGAATGCCAAAAAAGATTTTGATGAAAAGGCGGCCTATTCTTTAGTTCAAAAAGAAAGCTTGTTTGCTGGATTAAAAGTAGAGGTGGATATTCATCAACGTTGGATGATTATTGAGTCAAATGCAGGTGTGGAATTTAATCAACCACTTGGCAGATGTGTGTACCTTCATCACGTCGATAATATTGAGCAAATATTGCCTTATGTTCAAAAAAATAAGACGCAAACCATATCTATTTTTCCTTGGGAGTCATCATTTAAATATCGAGATGCGTTAGCATTAAAAGGTGCGGAAAGGATTGTAGAAGCAGGAATGAATAACATATTTCGAGTTGGTGGATCTCATGACGGAATGAGACCGTTGCAACGATTAGTGACATATATTTCTCATGAAAGGCCATCTAACTATACGGCTAAGGATGTTGCGGTTGAAATAGAACAGACTCGATTCCTGGAAGAAGATAAGTTCCTTGTATTTGTCCCATAA
Protein Sequence
MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKNIIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWISMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSILRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEIMQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVCFYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESLFAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIFPWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAKDVAVEIEQTRFLEEDKFLVFVP
AmpR bla beta-lactamase 2653..3514 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCGCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
T7 promoter 35..54 Fwd
DNA Sequence
TAATACGACTCACTATAGG
AmpR promoter bla 2548..2653 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 2
Label Gene Product Position Strand
f1 ori 1761..2217 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori 3684..4273 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA