pVoPo-mNG

187843 Synthetic Bacterial Expression

Plasmid Information

Purpose
Constitutive mNeonGreen-expression vector for Fusobacterium nucleatum
Depositor
Jörg Vogel
Article
Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119
Insert
mNeonGreen
Available Since
Oct. 10, 2023
Availability
Available

Sequence Information

Length
4,588 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
WELQut site — WELQut protease recognition and cleavag… 219..231 Rev
DNA Sequence
TGGGAACTCCAG
Protein Sequence
WELQ
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 3283..3292 Fwd
DNA Sequence
ACCTCCTTA
rep_origin 1
Label Gene Product Position Strand
ori — — 941..1529 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTTAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 809..844 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT