pVoPo-mSc

187842 Synthetic Bacterial Expression

Plasmid Information

Purpose
Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum
Depositor
Jörg Vogel
Article
Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119
Insert
mScarlet-I
Available Since
Oct. 10, 2023
Availability
Available

Sequence Information

Length
4,523 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
WELQut site — WELQut protease recognition and cleavag… 4292..4304 Rev
DNA Sequence
TGGGAACTCCAG
Protein Sequence
WELQ
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 2768..2777 Fwd
DNA Sequence
ACCTCCTTA
rep_origin 1
Label Gene Product Position Strand
ori — — 1409..1998 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 359..394 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT