pVoPo-mCh

187841 Synthetic Bacterial Expression

Plasmid Information

Purpose
Constitutive mCherry-expression vector for Fusobacterium nucleatum
Depositor
Jörg Vogel
Article
Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119
Insert
mCherry
Available Since
Sept. 7, 2023
Availability
Available

Sequence Information

Length
4,535 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
WELQut site — WELQut protease recognition and cleavag… 4304..4316 Rev
DNA Sequence
TGGGAACTCCAG
Protein Sequence
WELQ
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 2780..2789 Fwd
DNA Sequence
ACCTCCTTA
rep_origin 1
Label Gene Product Position Strand
ori — — 1421..2010 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 359..394 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT