pVoPo-GFP

187840 Synthetic Bacterial Expression

Plasmid Information

Purpose
Constitutive GFP-expression vector for Fusobacterium nucleatum
Depositor
Jörg Vogel
Article
Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119
Insert
sfGFP
Available Since
July 18, 2024
Availability
Available

Sequence Information

Length
5,879 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
superfolder GFP — GFP variant that folds robustly even wh… 491..1205 Fwd
DNA Sequence
ATGAGAAAAGGAGAAGAACTTTTTACAGGTGTTGTACCAATATTAGTTGAATTAGATGGAGATGTTAATGGTCATAAATTTTCAGTTAGAGGAGAAGGAGAAGGTGATGCAACTAATGGAAAATTAACTCTTAAGTTTATATGTACAACAGGAAAATTACCTGTACCATGGCCTACTTTAGTAACTACACTAACATATGGAGTTCAATGTTTTGCTAGATATCCAGATCATATGAAACAACATGACTTTTTCAAATCTGCAATGCCAGAAGGATATGTTCAAGAAAGAACAATAAGTTTTAAAGATGATGGTACATATAAAACTAGAGCAGAAGTTAAATTTGAAGGAGATACTTTAGTTAATAGAATAGAGTTAAAAGGAATTGACTTTAAAGAAGATGGAAATATTTTAGGACATAAACTTGAGTATAACTTTAATTCTCATAATGTTTACATTACTGCTGATAAACAAAAGAATGGTATAAAAGCTAATTTCAAGATAAGACATAATGTAGAAGATGGTTCTGTACAATTAGCAGATCATTATCAGCAAAATACACCTATTGGAGATGGTCCTGTATTATTACCAGATAATCACTATCTTTCAACTCAATCAGTACTAAGTAAAGATCCTAATGAAAAAAGAGATCATATGGTTTTGTTAGAATTTGTTACAGCTGCAGGAATTACTCATGGAATGGATGAATTGTATAAA
Protein Sequence
MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
WELQut site — WELQut protease recognition and cleavag… 5648..5660 Rev
DNA Sequence
TGGGAACTCCAG
Protein Sequence
WELQ
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 4124..4133 Fwd
DNA Sequence
ACCTCCTTA
rep_origin 1
Label Gene Product Position Strand
ori — — 1427..2016 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 359..394 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT