pEasyCas9

184909 Streptococcus pyogenes Yeast Expression

Plasmid Information

Purpose
Expresses the Streptococcus pyogenes Cas9 protein
Depositor
Jeferson Gross
Article
Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
Insert
Cas9
Available Since
Jan. 9, 2023
Availability
Available

Sequence Information

Length
9,549 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 344..1154 Fwd
DNA Sequence
ATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
Cas9 Cas9 (Csn1) endonuclease from the Strep… 2045..6149 Fwd
DNA Sequence
ATGGACAAGAAGTACTCTATTGGTTTGGACATTGGTACCAACTCTGTTGGTTGGGCTGTTATTACCGACGAATACAAGGTTCCATCTAAGAAGTTCAAGGTTTTGGGTAACACCGACAGACACTCTATTAAGAAGAACTTGATTGGTGCTTTGTTGTTCGACTCTGGTGAAACCGCTGAAGCTACCAGATTGAAGAGAACCGCTAGAAGAAGATACACCAGAAGAAAGAACAGAATTTGTTACTTGCAAGAAATTTTCTCTAACGAAATGGCTAAGGTTGACGACTCTTTCTTCCACAGATTGGAAGAATCTTTCTTGGTTGAAGAAGACAAGAAGCACGAAAGACACCCAATTTTCGGTAACATTGTTGACGAAGTTGCTTACCACGAAAAGTACCCAACCATTTACCACTTGAGAAAGAAGTTGGTTGACTCTACCGACAAGGCTGACTTGAGATTGATTTACTTGGCTTTGGCTCACATGATTAAGTTCAGAGGTCACTTCTTGATTGAAGGTGACTTGAACCCAGACAACTCTGACGTTGACAAGTTGTTCATTCAATTGGTTCAAACCTACAACCAATTGTTCGAAGAAAACCCAATTAACGCTTCTGGTGTTGACGCTAAGGCTATTTTGTCTGCTAGATTGTCTAAGTCTAGAAGATTGGAAAACTTGATTGCTCAATTGCCAGGTGAAAAGAAGAACGGTTTGTTCGGTAACTTGATTGCTTTGTCTTTGGGTTTGACCCCAAACTTCAAGTCTAACTTCGACTTGGCTGAAGACGCTAAGTTGCAATTGTCTAAGGACACCTACGACGACGACTTGGACAACTTGTTGGCTCAAATTGGTGACCAATACGCTGACTTGTTCTTGGCTGCTAAGAACTTGTCTGACGCTATTTTGTTGTCTGACATTTTGAGAGTTAACACCGAAATTACCAAGGCTCCATTGTCTGCTTCTATGATTAAGAGATACGACGAACACCACCAAGACTTGACCTTGTTGAAGGCTTTGGTTAGACAACAATTGCCAGAAAAGTACAAGGAAATTTTCTTCGACCAATCTAAGAACGGTTACGCTGGTTACATTGACGGTGGTGCTTCTCAAGAAGAATTCTACAAGTTCATTAAGCCAATTTTGGAAAAGATGGACGGTACCGAAGAATTGTTGGTTAAGTTGAACAGAGAAGACTTGTTGAGAAAGCAAAGAACCTTCGACAACGGTTCTATTCCACACCAAATTCACTTGGGTGAATTGCACGCTATTTTGAGAAGACAAGAAGACTTCTACCCATTCTTGAAGGACAACAGAGAAAAGATTGAAAAGATTTTGACCTTCAGAATTCCATACTACGTTGGTCCATTGGCTAGAGGTAACTCTAGATTCGCTTGGATGACCAGAAAGTCTGAAGAAACCATTACCCCATGGAACTTCGAAGAAGTTGTTGACAAGGGTGCTTCTGCTCAATCTTTCATTGAAAGAATGACCAACTTCGACAAGAACTTGCCAAACGAAAAGGTTTTGCCAAAGCACTCTTTGTTGTACGAATACTTCACCGTTTACAACGAATTGACCAAGGTTAAGTACGTTACCGAAGGTATGAGAAAGCCAGCTTTCTTGTCTGGTGAACAAAAGAAGGCTATTGTTGACTTGTTGTTCAAGACCAACAGAAAGGTTACCGTTAAGCAATTGAAGGAAGACTACTTCAAGAAGATTGAATGTTTCGACTCTGTTGAAATTTCTGGTGTTGAAGACAGATTCAACGCTTCTTTGGGTACCTACCACGACTTGTTGAAGATTATTAAGGACAAGGACTTCTTGGACAACGAAGAAAACGAAGACATTTTGGAAGACATTGTTTTGACCTTGACCTTGTTCGAAGACAGAGAAATGATTGAAGAAAGATTGAAGACCTACGCTCACTTGTTCGACGACAAGGTTATGAAGCAATTGAAGAGAAGAAGATACACCGGTTGGGGTAGATTGTCTAGAAAGTTGATTAACGGTATTAGAGACAAGCAATCTGGTAAGACCATTTTGGACTTCTTGAAGTCTGACGGTTTCGCTAACAGAAACTTCATGCAATTGATTCACGACGACTCTTTGACCTTCAAGGAAGACATTCAAAAGGCTCAAGTTTCTGGTCAAGGTGACTCTTTGCACGAACACATTGCTAACTTGGCTGGTTCTCCAGCTATTAAGAAGGGTATTTTGCAAACCGTTAAGGTTGTTGACGAATTGGTTAAGGTTATGGGTAGACACAAGCCAGAAAACATTGTTATTGAAATGGCTAGAGAAAACCAAACCACCCAAAAGGGTCAAAAGAACTCTAGAGAAAGAATGAAGAGAATTGAAGAAGGTATTAAGGAATTGGGTTCTCAAATTTTGAAGGAACACCCAGTTGAAAACACCCAATTGCAAAACGAAAAGTTGTACTTGTACTACTTGCAAAACGGTAGAGACATGTACGTTGACCAAGAATTGGACATTAACAGATTGTCTGACTACGACGTTGACCACATTGTTCCACAATCTTTCTTGAAGGACGACTCTATTGACAACAAGGTTTTGACCAGATCTGACAAGAACAGAGGTAAGTCTGACAACGTTCCATCTGAAGAAGTTGTTAAGAAGATGAAGAACTACTGGAGACAATTGTTGAACGCTAAGTTGATTACCCAAAGAAAGTTCGACAACTTGACCAAGGCTGAAAGAGGTGGTTTGTCTGAATTGGACAAGGCTGGTTTCATTAAGAGACAATTGGTTGAAACCAGACAAATTACCAAGCACGTTGCTCAAATTTTGGACTCTAGAATGAACACCAAGTACGACGAAAACGACAAGTTGATTAGAGAAGTTAAGGTTATTACCTTGAAGTCTAAGTTGGTTTCTGACTTCAGAAAGGACTTCCAATTCTACAAGGTTAGAGAAATTAACAACTACCACCACGCTCACGACGCTTACTTGAACGCTGTTGTTGGTACCGCTTTGATTAAGAAGTACCCAAAGTTGGAATCTGAATTCGTTTACGGTGACTACAAGGTTTACGACGTTAGAAAGATGATTGCTAAGTCTGAACAAGAAATTGGTAAGGCTACCGCTAAGTACTTCTTCTACTCTAACATTATGAACTTCTTCAAGACCGAAATTACCTTGGCTAACGGTGAAATTAGAAAGAGACCATTGATTGAAACCAACGGTGAAACCGGTGAAATTGTTTGGGACAAGGGTAGAGACTTCGCTACCGTTAGAAAGGTTTTGTCTATGCCACAAGTTAACATTGTTAAGAAGACCGAAGTTCAAACCGGTGGTTTCTCTAAGGAATCTATTTTGCCAAAGAGAAACTCTGACAAGTTGATTGCTAGAAAGAAGGACTGGGACCCAAAGAAGTACGGTGGTTTCGACTCTCCAACCGTTGCTTACTCTGTTTTGGTTGTTGCTAAGGTTGAAAAGGGTAAGTCTAAGAAGTTGAAGTCTGTTAAGGAATTGTTGGGTATTACCATTATGGAAAGATCTTCTTTCGAAAAGAACCCAATTGACTTCTTGGAAGCTAAGGGTTACAAGGAAGTTAAGAAGGACTTGATTATTAAGTTGCCAAAGTACTCTTTGTTCGAATTGGAAAACGGTAGAAAGAGAATGTTGGCTTCTGCTGGTGAATTGCAAAAGGGTAACGAATTGGCTTTGCCATCTAAGTACGTTAACTTCTTGTACTTGGCTTCTCACTACGAAAAGTTGAAGGGTTCTCCAGAAGACAACGAACAAAAGCAATTGTTCGTTGAACAACACAAGCACTACTTGGACGAAATTATTGAACAAATTTCTGAATTCTCTAAGAGAGTTATTTTGGCTGACGCTAACTTGGACAAGGTTTTGTCTGCTTACAACAAGCACAGAGACAAGCCAATTAGAGAACAAGCTGAAAACATTATTCACTTGTTCACCTTGACCAACTTGGGTGCTCCAGCTGCTTTCAAGTACTTCGACACCACCATTGACAGAAAGAGATACACCTCTACCAAGGAAGTTTTGGACGCTACCTTGATTCACCAATCTATTACCGGTTTGTACGAAACCAGAATTGACTTGTCTCAATTGGGTGGTGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS nuclear localization signal of SV40 (si… 6161..6182 Fwd
DNA Sequence
CCAAAGAAGAAGAGAAAGGTT
Protein Sequence
PKKKRKV
AmpR bla beta-lactamase 7214..8075 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
TEF promoter 0..344 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
AmpR promoter bla 8075..8180 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 1
Label Gene Product Position Strand
FRT 9169..9217 Rev
DNA Sequence
GAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
ori 6455..7044 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
2u ori 8206..9549 Fwd
DNA Sequence
AACGAAGCATCTGTGCTTCATTTTGTAGAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAAAGCGCTATTTTACCAACGAAGAATCTGTGCTTCATTTTTGTAAAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAGAGCGCTATTTTACCAACAAAGAATCTATACTTCTTTTTTGTTCTACAAAAATGCATCCCGAGAGCGCTATTTTTCTAACAAAGCATCTTAGATTACTTTTTTTCTCCTTTGTGCGCTCTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTTAGAAGAAGGCTACTTTGGTGTCTATTTTCTCTTCCATAAAAAAAGCCTGACTCCACTTCCCGCGTTTACTGATTACTAGCGAAGCTGCGGGTGCATTTTTTCAAGATAAAGGCATCCCCGATTATATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGCGTTGATGATTCTTCATTGGTCAGAAAATTATGAACGGTTTCTTCTATTTTGTCTCTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTCACTCTATGAATAGTTCTTACTACAATTTTTTTGTCTAAAGAGTAATACTAGAGATAAACATAAAAAATGTAGAGGTCGAGTTTAGATGCAAGTTCAAGGAGCGAAAGGTGGATGGGTAGGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCAATATTTTAGTAGCTCGTTACAGTCCGGTGCGTTTTTGGTTTTTTGAAAGTGCGTCTTCAGAGCGCTTTTGGTTTTCAAAAGCGCTCTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTGCGCACATACAGCTCACTGTTCACGTCGCACCTATATCTGCGTGTTGCCTGTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATGCGTACTTATATGCGTCTATTTATGTAGGATGAAAGGTAGTCTAGTACCTCCTGTGATATTATCCCATTCCATGCGGGGTATCGTATGCTTCCTTCAGCACTACCCTTTAGCTGTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCTATCATTTCCTTTGATATTGGATC
terminator 2
Label Gene Product Position Strand
TEF terminator 1159..1357 Fwd
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG
CYC1 terminator S. cerevisiae CYC1 6197..6445 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC