pDS2142

184273 Synthetic Yeast Expression

Plasmid Information

Purpose
Tet-off genome insertion system
Depositor
Dominique Sanglard
Article
Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.
Insert
CatTA
Promoter
TDH3
Available Since
May 12, 2022
Availability
Available

Sequence Information

Length
10,071 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 2113..3139 Fwd
DNA Sequence
ATGAAAAAACCAGAATTGACTGCTACTTCTGTTGAAAAATTTTTGATTGAAAAATTTGATTCTGTTTCTGATTTGATGCAATTGTCTGAAGGTGAAGAATCAAGAGCTTTTTCTTTTGATGTTGGTGGTAGAGGTTATGTTTTGAGAGTTAATTCTTGTGCTGATGGTTTTTATAAAGATAGATATGTTTATAGACATTTTGCTTCTGCTGCTTTGCCAATTCCAGAAGTTTTGGATATTGGTGAATTTTCTGAATCTTTGACTTATTGTATTTCAAGAAGAGCTCAAGGTGTTACTTTGCAAGATTTGCCAGAAACTGAATTGCCAGCTGTTTTGCAACCAGTTGCTGAAGCTATGGATGCTATTGCTGCTGCTGATTTGTCTCAAACTTCTGGTTTTGGTCCATTTGGTCCACAAGGTATTGGTCAATATACTACTTGGAGAGATTTTATTTGTGCTATTGCTGATCCACATGTTTATCATTGGCAAACTGTTATGGATGATACTGTTTCTGCTTCTGTTGCTCAAGCATTGGATGAATTGATGTTGTGGGCTGAAGATTGTCCAGAAGTTAGACATTTGGTTCATGCTGATTTTGGTTCTAATAATGTTTTGACTGATAATGGTAGAATTACTGCTGTTATTGATTGGTCTGAAGCTATGTTTGGTGATTCTCAATATGAAGTTGCTAATATTTTTTTTTGGAGACCATGGTTGGCATGTATGGAACAACAAACTAGATATTTTGAAAGAAGACATCCAGAATTGGCTGGTTCTCCAAGATTGAGAGCTTATATGTTGAGAATTGGTTTGGATCAATTGTATCAATCTTTGGTTGATGGTAATTTTGATGATGCTGCTTGGGCTCAAGGTAGATGTGATGCTATTGTTAGATCAGGTGCTGGTACTGTTGGTAGAACTCAAATTGCTAGAAGATCAGCTGCTGTTTGGACTGATGGTTGTGTTGAAGTTTTGGCTGATTCTGGTAATAGAAGACCATCTACTAGACCAAGAGCTAAAGAATAA
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
AmpR bla beta-lactamase 6744..7605 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 4
Label Gene Product Position Strand
T3 promoter 5544..5563 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter 5631..5662 Rev
DNA Sequence
TTTACACTTTATGCTCCCGGCTCGTATGTTG
AmpR promoter bla 7605..7710 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
T7 promoter 8358..8377 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator 5607..5624 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 5676..5698 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
ori 5985..6574 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
f1 ori 7735..8191 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT