pSL103-SpCas9-FLAG-3XNLS

182032 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
Depositor
Steven Lin
Article
Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
Insert
Streptococcus pyogenes cas9 (cas9 Synthetic)
Tags
12X His tag, 3XNLS, FLAG, and MBP
Promoter
T7
Available Since
May 23, 2022
Availability
Available

Sequence Information

Length
10,759 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 15
Label Gene Product Position Strand
lacI lacI lac repressor 655..1738 Rev
DNA Sequence
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGTATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA
Protein Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
MBP malE (mutated) maltose binding protein from E. coli 2211..3315 Fwd
DNA Sequence
ATGGGTAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACT
Protein Sequence
MGKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
TEV site tobacco etch virus (TEV) protease recog… 3369..3390 Fwd
DNA Sequence
GAAAACCTGTACTTCCAAGGA
Protein Sequence
ENLYFQG
Cas9 Cas9 (Csn1) endonuclease from the Strep… 3390..7494 Fwd
DNA Sequence
ATGGACAAAAAATACAGCATCGGTCTGGATATTGGCACCAATAGCGTTGGTTGGGCAGTTATTACCGATGAATATAAAGTGCCGAGCAAAAAATTCAAAGTGCTGGGTAATACCGATCGCCACAGCATCAAAAAAAACCTGATTGGTGCACTGCTGTTTGATAGCGGTGAAACCGCAGAAGCAACCCGTCTGAAACGTACCGCACGTCGTCGTTATACCCGTCGTAAAAATCGTATTTGTTACCTGCAAGAGATCTTTAGCAACGAAATGGCCAAAGTGGATGATAGCTTTTTTCATCGTCTGGAAGAATCCTTTCTGGTGGAAGAAGATAAAAAACACGAACGCCATCCGATTTTTGGCAACATTGTTGATGAAGTGGCCTACCATGAAAAATATCCGACCATTTATCATCTGCGCAAAAAACTGGTTGATAGCACCGATAAAGCCGATCTGCGTCTGATTTATCTGGCACTGGCACATATGATCAAATTTCGTGGTCATTTTCTGATCGAAGGTGATCTGAATCCGGATAATAGTGATGTGGATAAACTGTTCATTCAGCTGGTGCAGACCTATAATCAGCTGTTTGAAGAAAATCCGATTAATGCCAGCGGTGTTGATGCAAAAGCAATTCTGAGCGCACGTCTGAGCAAAAGCCGTCGCCTGGAAAATCTGATTGCACAGCTGCCTGGTGAGAAAAAAAACGGTCTGTTTGGTAATCTGATCGCACTGAGCCTGGGTCTGACCCCGAACTTTAAATCAAATTTTGATCTGGCCGAAGATGCCAAACTGCAGCTGAGCAAAGATACCTATGATGATGATCTGGATAATCTGCTGGCACAGATTGGTGATCAGTATGCAGACCTGTTTCTGGCAGCGAAAAATCTGAGTGATGCAATTCTGCTGAGCGATATTCTGCGTGTTAATACCGAAATTACCAAAGCACCGCTGAGCGCAAGCATGATTAAACGTTATGATGAACATCATCAGGATCTGACCCTGCTGAAAGCACTGGTTCGTCAGCAGCTGCCGGAAAAATACAAAGAAATCTTTTTCGACCAGAGCAAAAATGGCTATGCCGGTTATATTGATGGTGGTGCAAGCCAAGAAGAGTTCTATAAATTCATTAAACCGATCCTGGAAAAAATGGACGGCACCGAAGAACTGCTGGTTAAACTGAATCGTGAAGATCTGCTGCGTAAACAGCGTACCTTTGATAATGGTAGCATTCCGCATCAGATTCATCTGGGTGAACTGCATGCCATTCTGCGTCGTCAAGAAGATTTTTATCCGTTCCTGAAAGATAACCGCGAAAAAATCGAGAAAATTCTGACCTTTCGCATCCCGTATTATGTGGGTCCGCTGGCACGTGGTAATAGCCGTTTTGCATGGATGACCCGTAAAAGCGAAGAAACCATTACCCCGTGGAATTTTGAAGAGGTTGTTGATAAAGGTGCAAGCGCACAGAGCTTTATTGAACGTATGACCAACTTCGATAAAAACCTGCCGAATGAAAAAGTGCTGCCGAAACATAGCCTGCTGTATGAATATTTCACCGTGTATAATGAGCTGACCAAAGTGAAATATGTGACCGAAGGTATGCGTAAACCGGCATTTCTGAGCGGTGAACAGAAAAAAGCGATTGTTGATCTGCTGTTTAAAACCAATCGTAAAGTGACCGTGAAACAACTGAAAGAAGATTACTTCAAAAAAATCGAATGCTTTGATAGCGTCGAAATCAGCGGTGTGGAAGATCGTTTTAATGCAAGCCTGGGCACCTATCACGATCTGCTGAAAATCATCAAAGATAAAGATTTTCTGGACAACGAAGAGAACGAGGACATTCTGGAAGATATTGTGCTGACCCTGACCCTGTTTGAGGATCGTGAAATGATTGAAGAACGCCTGAAAACCTATGCCCACCTGTTTGATGATAAAGTGATGAAACAACTGAAACGCCGTCGTTATACCGGTTGGGGTCGTCTGAGCCGTAAACTGATTAATGGTATTCGTGATAAACAGAGCGGCAAAACCATTCTGGATTTTCTGAAAAGTGATGGCTTTGCCAATCGCAATTTTATGCAGCTGATTCATGATGATAGCCTGACCTTTAAAGAGGATATTCAGAAAGCACAGGTTAGCGGTCAGGGTGATAGTCTGCATGAACATATTGCAAATCTGGCAGGTAGTCCGGCAATCAAAAAAGGTATTCTGCAGACCGTTAAAGTGGTTGATGAACTGGTTAAAGTTATGGGTCGTCATAAACCGGAAAACATCGTTATTGAAATGGCACGTGAAAATCAGACCACCCAGAAAGGCCAGAAAAATAGCCGTGAACGCATGAAACGTATTGAAGAAGGTATTAAAGAACTGGGCAGCCAGATTCTGAAAGAACATCCGGTTGAAAATACCCAGCTGCAGAACGAAAAACTGTATCTGTATTACCTGCAGAATGGTCGTGACATGTATGTTGATCAAGAACTGGATATTAATCGCCTGAGCGATTATGATGTCGATCATATTGTTCCGCAGTCCTTTCTGAAAGATGATAGCATTGATAACAAAGTTCTGACCCGCAGCGACAAAAATCGTGGTAAAAGTGATAATGTTCCGAGCGAAGAGGTGGTGAAAAAAATGAAAAACTATTGGCGTCAACTGCTGAACGCCAAACTGATCACACAGCGCAAATTTGATAATCTGACAAAAGCAGAACGTGGCGGTCTGAGCGAGCTGGATAAAGCAGGTTTTATCAAACGTCAGCTGGTTGAAACCCGTCAGATCACAAAACATGTTGCACAGATTCTGGATAGCCGCATGAATACCAAATATGACGAAAACGATAAACTGATCCGCGAGGTTAAAGTGATTACCCTGAAAAGCAAACTGGTGAGCGATTTTCGTAAAGACTTCCAGTTTTACAAAGTGCGCGAAATCAACAATTATCATCACGCACATGATGCCTATCTGAATGCAGTTGTTGGCACCGCACTGATCAAAAAATACCCGAAACTGGAAAGCGAATTCGTGTATGGTGACTATAAAGTTTACGATGTGCGCAAAATGATCGCCAAAAGCGAACAAGAAATTGGTAAAGCAACCGCCAAATACTTCTTCTACAGCAATATCATGAACTTTTTCAAAACCGAAATCACCCTGGCCAATGGTGAAATTCGTAAACGTCCGCTGATCGAAACCAATGGCGAAACCGGTGAAATTGTTTGGGATAAAGGTCGTGATTTTGCCACCGTTCGTAAAGTTCTGTCAATGCCGCAGGTTAACATTGTCAAAAAAACTGAAGTTCAGACCGGTGGCTTTAGCAAAGAATCAATTCTGCCTAAACGCAATAGCGACAAACTGATTGCCCGTAAAAAAGATTGGGACCCGAAAAAATACGGTGGTTTTGATAGCCCGACCGTTGCATATAGCGTTCTGGTTGTTGCAAAAGTGGAAAAAGGTAAAAGCAAAAAACTGAAAAGCGTGAAAGAGCTGCTGGGCATTACCATTATGGAACGTAGCAGCTTTGAAAAAAACCCGATCGATTTCCTGGAAGCCAAAGGTTATAAAGAAGTGAAAAAAGATCTGATCATTAAACTGCCGAAATACTCCCTGTTCGAACTGGAAAATGGTCGTAAACGCATGCTGGCAAGTGCAGGCGAACTGCAGAAAGGTAATGAACTGGCACTGCCGAGTAAATACGTGAATTTTCTGTATCTGGCCAGCCACTATGAGAAACTGAAAGGTTCACCGGAAGATAACGAACAAAAACAACTGTTTGTGGAACAGCACAAACACTATCTGGATGAAATTATTGAGCAGATCAGCGAATTTAGCAAACGTGTTATTCTGGCAGATGCCAATCTGGACAAAGTACTGAGCGCATATAACAAACATCGTGACAAACCGATTCGTGAACAGGCCGAAAACATTATTCACCTGTTTACCCTGACCAATCTGGGTGCACCGGCAGCCTTTAAATACTTTGATACCACCATTGATCGCAAACGTTATACCAGCACCAAAGAAGTTCTGGACGCAACCCTGATTCATCAGAGCATTACCGGTCTGTATGAAACCCGCATTGATCTGAGTCAGCTGGGTGGTGAT
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
3xFLAG three tandem FLAG(R) epitope tags, foll… 7497..7563 Fwd
DNA Sequence
GATTATAAAGATCATGATGGCGATTACAAAGACCACGACATCGACTATAAAGATGACGATGATAAA
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
SV40 NLS nuclear localization signal of SV40 (si… 7566..7587 Fwd
DNA Sequence
CCGAAAAAAAAACGTAAAGTG
Protein Sequence
PKKKRKV
SV40 NLS nuclear localization signal of SV40 (si… 7593..7614 Fwd
DNA Sequence
CCTAAAAAAAAACGCAAAGTT
Protein Sequence
PKKKRKV
SV40 NLS nuclear localization signal of SV40 (si… 7620..7641 Fwd
DNA Sequence
CCGAAAAAAAAACGGAAAGTC
Protein Sequence
PKKKRKV
TEV site tobacco etch virus (TEV) protease recog… 7650..7671 Fwd
DNA Sequence
GAGAATTTATATTTTCAGAGT
Protein Sequence
ENLYFQS
10xHis 10xHis affinity tag 7683..7713 Fwd
DNA Sequence
CACCATCACCATCACCATCACCATCACCAT
Protein Sequence
HHHHHHHHHH
10xHis 10xHis affinity tag 7686..7716 Fwd
DNA Sequence
CATCACCATCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHHHH
10xHis 10xHis affinity tag 7689..7719 Fwd
DNA Sequence
CACCATCACCATCACCATCACCATCACCAC
Protein Sequence
HHHHHHHHHH
6xHis 6xHis affinity tag 7746..7764 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
KanR aph(3')-Ia aminoglycoside phosphotransferase 8462..9278 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
rop rop Rop protein, which maintains plasmids a… 10414..10606 Rev
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
promoter 2
Label Gene Product Position Strand
lacI promoter lacI 1738..1816 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter 2124..2143 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 2143..2168 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 2
Label Gene Product Position Strand
f1 ori 7914..8370 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAACTAGTAACGTTTACAATTT
ori 9399..9988 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator 7830..7878 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG