| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR | bla | beta-lactamase | 1172..2033 | Rev | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 4457..4514 | Fwd | |
|
DNA Sequence
GAAGAACTTTTGAGCAAGAATTATCATCTTGAGAACGAAGTGGCTCGTCTTAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 4529..4586 | Fwd | |
|
DNA Sequence
GAAGAACTGCTTTCAAAGAATTACCACCTGGAAAATGAGGTAGCTAGACTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 4601..4658 | Fwd | |
|
DNA Sequence
GAGGAGTTGCTGAGCAAAAATTATCATTTGGAGAACGAAGTAGCACGACTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5201..5258 | Fwd | |
|
DNA Sequence
GAGGAGTTACTCTCGAAAAATTATCATCTCGAAAACGAAGTGGCTCGGCTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5273..5330 | Fwd | |
|
DNA Sequence
GAAGAGCTATTATCTAAAAACTACCACCTCGAAAATGAGGTGGCACGCTTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5345..5402 | Fwd | |
|
DNA Sequence
GAAGAGCTACTATCCAAGAATTATCATCTTGAGAACGAGGTAGCGCGTTTGAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5627..5684 | Fwd | |
|
DNA Sequence
GAGGAACTACTTTCAAAGAATTACCACCTCGAAAACGAAGTAGCTCGATTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5699..5756 | Fwd | |
|
DNA Sequence
GAAGAATTACTGAGTAAAAATTATCATCTGGAAAATGAGGTAGCGAGACTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| GCN4_v4 | — | GCN4 peptide optimized to improve solub… | 5771..5828 | Fwd | |
|
DNA Sequence
GAAGAGTTGCTATCGAAAAATTATCATCTTGAGAACGAAGTTGCTAGGCTCAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
|
|||||
| hRluc | — | Renilla luciferase | 5861..6791 | Fwd | |
|
DNA Sequence
GCTTCCAAGGTGTACGACCCCGAGCAACGCAAACGCATGATCACTGGGCCTCAGTGGTGGGCTCGCTGCAAGCAAATGAACGTGCTGGACTCCTTCATCAACTACTATGATTCCGAGAAGCACGCCGAGAACGCCGTGATTTTTCTGCATGGTAACGCTGCCTCCAGCTACCTGTGGAGGCACGTCGTGCCTCACATCGAGCCCGTGGCTAGATGCATCATCCCTGATCTGATCGGAATGGGTAAGTCCGGCAAGAGCGGGAATGGCTCATATCGCCTCCTGGATCACTACAAGTACCTCACCGCTTGGTTCGAGCTGCTGAACCTTCCAAAGAAAATCATCTTTGTGGGCCACGACTGGGGGGCTTGTCTGGCCTTTCACTACTCCTACGAGCACCAAGACAAGATCAAGGCCATCGTCCATGCTGAGAGTGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCTGACATCGAGGAGGATATCGCCCTGATCAAGAGCGAAGAGGGCGAGAAAATGGTGCTTGAGAATAACTTCTTCGTCGAGACCATGCTCCCAAGCAAGATCATGCGGAAACTGGAGCCTGAGGAGTTCGCTGCCTACCTGGAGCCATTCAAGGAGAAGGGCGAGGTTAGACGGCCTACCCTCTCCTGGCCTCGCGAGATCCCTCTCGTTAAGGGAGGCAAGCCCGACGTCGTCCAGATTGTCCGCAACTACAACGCCTACCTTCGGGCCAGCGACGATCTGCCTAAGATGTTCATCGAGTCCGACCCTGGGTTCTTTTCCAACGCTATTGTCGAGGGAGCTAAGAAGTTCCCTAACACCGAGTTCGTGAAGGTGAAGGGCCTCCACTTCAGCCAGGAGGACGCTCCAGATGAAATGGGTAAGTACATCAAGAGCTTCGTGGAGCGCGTGCTGAAGAACGAGCAG
Protein Sequence
ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ
|
|||||
| DD | — | destabilization domain that can be stab… | 6791..7115 | Fwd | |
|
DNA Sequence
ATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGTGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTCGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACCGGAA
Protein Sequence
MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKPE
|
|||||
| DD-C | — | destabilization domain that can be stab… | 6794..7118 | Fwd | |
|
DNA Sequence
GGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGTGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTCGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACCGGAATAA
Protein Sequence
GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKPE
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| chimeric intron | — | — | 4183..4316 | Fwd | |
|
DNA Sequence
GTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| SV40 poly(A) signal | — | — | 8647..8782 | Fwd | |
|
DNA Sequence
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCAAAACTAGAATGCAGTGAAAAAAATGCCTTATTTGTGAAATTTGTGATGCTATTGCCTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac promoter | — | — | 59..90 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| AmpR promoter | bla | — | 2033..2138 | Rev | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| T7 promoter | — | — | 4360..4379 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 35..52 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 104..126 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| FRT | — | — | 3709..3757 | Rev | |
|
DNA Sequence
GAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
|
|||||
| tet operator | tetO | — | 3803..3822 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3839..3858 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3875..3894 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3911..3930 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3947..3966 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 3983..4002 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 4019..4038 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| FRT3 | — | — | 8889..8923 | Rev | |
|
DNA Sequence
GAAGTTCCTATTCTTCAAATAGTATAGGAACTTC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 413..1002 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||