pGal-Trans-Myc-HA-GFP11-KDEL

Plasmid Information

Purpose

ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Sequence Information

Length

6,959 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 6

Label	Gene	Product	Position	Strand
HygR	aph(4)-Ia	aminoglycoside phosphotransferase from …	0..6959	Fwd
DNA Sequence ATGAAGAAGCCCGAACTCACCGCTACCAGCGTTGAAAAATTTCTCATCGAGAAGTTCGACAGTGTGAGCGACCTGATGCAGTTGTCGGAGGGCGAAGAGAGCCGAGCCTTCAGCTTCGATGTCGGCGGACGCGGCTATGTACTGCGGGTGAATAGCTGCGCTGATGGCTTCTACAAAGACCGCTACGTGTACCGCCACTTCGCCAGCGCTGCACTACCCATCCCCGAAGTGTTGGACATCGGCGAGTTCAGCGAGAGCCTGACATACTGCATCAGTAGACGCGCCCAAGGCGTTACTCTCCAAGACCTCCCCGAAACAGAGCTGCCTGCTGTGTTACAGCCTGTCGCCGAAGCTATGGATGCTATTGCCGCCGCCGACCTCAGTCAAACCAGCGGCTTCGGCCCATTCGGGCCCCAAGGCATCGGCCAGTACACAACCTGGCGGGATTTCATTTGCGCCATTGCTGATCCCCATGTCTACCACTGGCAGACCGTGATGGACGACACCGTGTCCGCCAGCGTAGCTCAAGCCCTGGACGAACTGATGCTGTGGGCCGAAGACTGTCCCGAGGTGCGCCACCTCGTCCATGCCGACTTCGGCAGCAACAACGTCCTGACCGACAACGGCCGCATCACCGCCGTAATCGACTGGTCCGAAGCTATGTTCGGGGACAGTCAGTACGAGGTGGCCAACATCTTCTTCTGGCGGCCCTGGCTGGCTTGCATGGAGCAGCAGACTCGCTACTTCGAGCGCCGGCATCCCGAGCTGGCCGGCAGCCCTCGTCTGCGAGCCTACATGCTGCGCATCGGCCTGGATCAGCTCTACCAGAGCCTCGTGGACGGCAACTTCGACGATGCTGCCTGGGCTCAAGGCCGCTGCGATGCCATCGTCCGCAGCGGGGCCGGCACCGTCGGTCGCACACAAATCGCTCGCCGGAGCGCAGCCGTATGGACCGACGGCTGCGTCGAGGTGCTGGCCGACAGCGGCAACCGCCGGCCCAGTACACGACCGCGCGCTAAGGAGGTAGGTCGAGTTTAA Protein Sequence MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV
AmpR	bla	beta-lactamase	1601..2462	Rev
DNA Sequence ATGAGCATCCAACATTTTCGTGTCGCACTCATTCCCTTCTTTGCGGCATTTTGCTTGCCTGTTTTTGCACACCCCGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAACTGGGTGCAAGAGTGGGCTATATCGAACTGGATCTCAATAGCGGCAAGATCCTTGAGTCTTTCCGCCCCGAAGAACGATTCCCGATGATGAGCACTTTCAAAGTACTGCTATGTGGCGCGGTGTTGTCCCGTATAGACGCCGGGCAAGAGCAGCTTGGTCGCCGTATACACTACTCACAAAACGACTTGGTTGAGTACTCGCCGGTCACGGAAAAGCATCTTACGGATGGCATGACGGTAAGAGAATTGTGTAGTGCTGCCATTACCATGAGCGACAACACCGCGGCCAACTTACTTCTGACAACGATCGGAGGCCCTAAGGAGCTGACTGCATTTCTTCATAATATGGGTGATCATGTGACCCGGCTTGACCGCTGGGAACCAGAGTTGAACGAAGCCATACCGAACGACGAGCGTGATACCACGATGCCAGTAGCAATGGCCACAACTCTTCGGAAACTACTCACTGGCGAACTTCTTACTCTAGCATCACGACAGCAGCTCATAGACTGGATGGAGGCGGACAAAGTAGCAGGACCACTTCTTCGCTCGGCCCTCCCTGCTGGCTGGTTCATTGCTGACAAATCGGGGGCCGGTGAACGCGGCTCTCGCGGCATCATTGCTGCGCTGGGGCCTGATGGTAAGCCCTCACGAATCGTAGTGATCTACACGACGGGGAGTCAGGCCACTATGGACGAACGAAATAGGCAGATCGCTGAGATCGGTGCCTCACTGATCAAGCACTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
WELQut site	—	WELQut protease recognition and cleavag…	4202..4214	Rev
DNA Sequence TGGGAACTGCAA Protein Sequence WELQ
Myc	—	Myc (human c-Myc proto-oncogene) epitop…	5404..5434	Fwd
DNA Sequence GAGCAGAAACTCATCTCAGAAGAGGATCTG Protein Sequence EQKLISEEDL
HA	—	HA (human influenza hemagglutinin) epit…	5452..5479	Fwd
DNA Sequence TACCCATACGATGTTCCAGATTACGCT Protein Sequence YPYDVPDYA
GFP11	—	11th beta-strand of superfolder GFP	5518..5566	Fwd
DNA Sequence AGAGATCATATGGTTTTGCATGAATATGTTAATGCTGCTGGTATTACT Protein Sequence RDHMVLHEYVNAAGIT

RBS 1

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	393..402	Fwd
DNA Sequence TAAGGAGGT

enhancer 2

Label	Gene	Product	Position	Strand
CMV enhancer	—	—	2870..3250	Fwd
DNA Sequence GGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
SV40 enhancer	—	—	5948..6140	Fwd
DNA Sequence CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCC

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	5633..5755	Fwd
DNA Sequence TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

promoter 2

Label	Gene	Product	Position	Strand
CMV promoter	—	—	3250..3454	Fwd
DNA Sequence GTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
SV40 promoter	—	—	5948..6306	Fwd
DNA Sequence CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA

rep_origin 2

Label	Gene	Product	Position	Strand
ori	—	—	813..1402	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
SV40 ori	—	—	6156..6292	Fwd
DNA Sequence ATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC