pAT-WT-HA-GFP11 — PlasmidDB

Plasmid Information

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Sequence Information

Length

6,261 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
HygR	aph(4)-Ia	aminoglycoside phosphotransferase from …	140..1178	Fwd
DNA Sequence ATGAAGAAGCCCGAACTCACCGCTACCAGCGTTGAAAAATTTCTCATCGAGAAGTTCGACAGTGTGAGCGACCTGATGCAGTTGTCGGAGGGCGAAGAGAGCCGAGCCTTCAGCTTCGATGTCGGCGGACGCGGCTATGTACTGCGGGTGAATAGCTGCGCTGATGGCTTCTACAAAGACCGCTACGTGTACCGCCACTTCGCCAGCGCTGCACTACCCATCCCCGAAGTGTTGGACATCGGCGAGTTCAGCGAGAGCCTGACATACTGCATCAGTAGACGCGCCCAAGGCGTTACTCTCCAAGACCTCCCCGAAACAGAGCTGCCTGCTGTGTTACAGCCTGTCGCCGAAGCTATGGATGCTATTGCCGCCGCCGACCTCAGTCAAACCAGCGGCTTCGGCCCATTCGGGCCCCAAGGCATCGGCCAGTACACAACCTGGCGGGATTTCATTTGCGCCATTGCTGATCCCCATGTCTACCACTGGCAGACCGTGATGGACGACACCGTGTCCGCCAGCGTAGCTCAAGCCCTGGACGAACTGATGCTGTGGGCCGAAGACTGTCCCGAGGTGCGCCACCTCGTCCATGCCGACTTCGGCAGCAACAACGTCCTGACCGACAACGGCCGCATCACCGCCGTAATCGACTGGTCCGAAGCTATGTTCGGGGACAGTCAGTACGAGGTGGCCAACATCTTCTTCTGGCGGCCCTGGCTGGCTTGCATGGAGCAGCAGACTCGCTACTTCGAGCGCCGGCATCCCGAGCTGGCCGGCAGCCCTCGTCTGCGAGCCTACATGCTGCGCATCGGCCTGGATCAGCTCTACCAGAGCCTCGTGGACGGCAACTTCGACGATGCTGCCTGGGCTCAAGGCCGCTGCGATGCCATCGTCCGCAGCGGGGCCGGCACCGTCGGTCGCACACAAATCGCTCGCCGGAGCGCAGCCGTATGGACCGACGGCTGCGTCGAGGTGCTGGCCGACAGCGGCAACCGCCGGCCCAGTACACGACCGCGCGCTAAGGAGGTAGGTCGAGTTTAA Protein Sequence MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV
AmpR	bla	beta-lactamase	2364..3225	Rev
DNA Sequence ATGAGCATCCAACATTTTCGTGTCGCACTCATTCCCTTCTTTGCGGCATTTTGCTTGCCTGTTTTTGCACACCCCGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAACTGGGTGCAAGAGTGGGCTATATCGAACTGGATCTCAATAGCGGCAAGATCCTTGAGTCTTTCCGCCCCGAAGAACGATTCCCGATGATGAGCACTTTCAAAGTACTGCTATGTGGCGCGGTGTTGTCCCGTATAGACGCCGGGCAAGAGCAGCTTGGTCGCCGTATACACTACTCACAAAACGACTTGGTTGAGTACTCGCCGGTCACGGAAAAGCATCTTACGGATGGCATGACGGTAAGAGAATTGTGTAGTGCTGCCATTACCATGAGCGACAACACCGCGGCCAACTTACTTCTGACAACGATCGGAGGCCCTAAGGAGCTGACTGCATTTCTTCATAATATGGGTGATCATGTGACCCGGCTTGACCGCTGGGAACCAGAGTTGAACGAAGCCATACCGAACGACGAGCGTGATACCACGATGCCAGTAGCAATGGCCACAACTCTTCGGAAACTACTCACTGGCGAACTTCTTACTCTAGCATCACGACAGCAGCTCATAGACTGGATGGAGGCGGACAAAGTAGCAGGACCACTTCTTCGCTCGGCCCTCCCTGCTGGCTGGTTCATTGCTGACAAATCGGGGGCCGGTGAACGCGGCTCTCGCGGCATCATTGCTGCGCTGGGGCCTGATGGTAAGCCCTCACGAATCGTAGTGATCTACACGACGGGGAGTCAGGCCACTATGGACGAACGAAATAGGCAGATCGCTGAGATCGGTGCCTCACTGATCAAGCACTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
HA	—	HA (human influenza hemagglutinin) epit…	5538..5565	Fwd
DNA Sequence TACCCATACGATGTTCCAGATTACGCT Protein Sequence YPYDVPDYA
GFP11	—	11th beta-strand of superfolder GFP	5604..5652	Fwd
DNA Sequence AGAGATCATATGGTTTTGCATGAATATGTTAATGCTGCTGGTATTACT Protein Sequence RDHMVLHEYVNAAGIT

RBS 1

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	1156..1165	Fwd
DNA Sequence TAAGGAGGT

enhancer 2

Label	Gene	Product	Position	Strand
CMV enhancer	—	—	3633..4013	Fwd
DNA Sequence GGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
SV40 enhancer	—	—	6013..6205	Fwd
DNA Sequence CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCC

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	5698..5820	Fwd
DNA Sequence TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

promoter 2

Label	Gene	Product	Position	Strand
SV40 promoter	—	—	0..6261	Fwd
DNA Sequence CTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA
CMV promoter	—	—	4013..4217	Fwd
DNA Sequence GTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

rep_origin 2

Label	Gene	Product	Position	Strand
SV40 ori	—	—	0..6261	Fwd
DNA Sequence ATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC
ori	—	—	1576..2165	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA