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pST39-His/Vps27(345-440) - Hse(275-375)
pST39-His/Vps27(345-440) - Hse(275-375)
17638
Saccharomyces cerevisiae
Bacterial Expression
Plasmid Information
Depositor
James Hurley
Article
Prag et al Dev Cell. 2007 Jun . 12(6):973-86.
Insert
His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)
Available Since
March 28, 2008
Availability
Available
Sequence Information
Length
697 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org
Features
CDS
2
Label
Gene
Product
Position
Strand
6xHis
—
6xHis affinity tag
150..168
Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site
—
tobacco etch virus (TEV) protease recog…
189..210
Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG