ALPaGA

175272 Escherichia coli Bacterial Expression

Plasmid Information

Purpose
Lactate biosensor in anaerobic conditions
Depositor
Jerome Bonnet
Article
Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021
Insert
LldR_Synthetic_promoter_sfGFP
Available Since
Dec. 7, 2021
Availability
Available

Sequence Information

Length
5,404 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
superfolder GFP GFP variant that folds robustly even wh… 1213..1930 Fwd
DNA Sequence
ATGTCAAAAGGAGAAGAACTTTTTACAGGTGTAGTACCTATCTTGGTTGAATTGGATGGTGATGTTAACGGTCACAAATTTTCTGTACGTGGTGAAGGTGAAGGTGATGCAACTAACGGTAAATTGACACTTAAATTCATTTGTACAACTGGAAAACTTCCTGTTCCTTGGCCTACTCTTGTTACAACATTGACATATGGAGTACAATGTTTTTCACGTTATCCTGATCATATGAAACGTCACGATTTTTTTAAATCTGCTATGCCAGAAGGTTATGTACAAGAACGTACAATTTCATTTAAAGATGACGGAACATATAAAACACGTGCTGAAGTAAAATTCGAAGGTGACACTCTTGTTAATCGTATCGAATTGAAAGGAATCGATTTCAAAGAAGATGGTAACATTTTGGGACACAAACTTGAATACAACTTCAACTCTCATAATGTTTATATCACAGCTGACAAACAAAAAAACGGTATTAAAGCTAATTTTAAAATTCGTCACAATGTTGAAGATGGATCTGTTCAATTGGCTGATCATTATCAACAAAATACACCAATCGGAGACGGACCAGTATTGCTTCCAGATAACCACTACCTTTCTACTCAATCAGTTCTTTCAAAAGATCCTAACGAAAAACGTGACCATATGGTACTTCTTGAATTTGTTACAGCAGCAGGTATCACTCACGGTATGGACGAACTTTATAAATAA
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
KanR aph(3')-Ia aminoglycoside phosphotransferase 2296..3112 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCCGTCCGCGCTTAAACTCCAACATGGACGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCCGTCTCAACTGGCTGACGGAGTTTATGCCTCTCCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCGTGGTTACTCACCACCGCGATTCCTGGGAAAACAGCCTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCCGTGTTCCTGCGCCGGTTACATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGTGTATTTCGTCTTGCTCAGGCGCAATCACGCATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCACAAGCTCTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGGGTCGGAATCGCAGACCGTTACCAGGACCTTGCCATTCTTTGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
Rep101 rep101 RepA protein needed for replication wit… 3469..4420 Rev
DNA Sequence
ATGTCTGAATTAGTTGTTTTCAAAGCAAATGAACTAGCGATTAGTCGCTATGACTTAACGGAGCATGAAACCAAGCTAATTTTATGCTGTGTGGCACTACTCAACCCCACGATTGAAAACCCTACAAGGAAAGAACGGACGGTATCGTTCACTTATAACCAATACGCTCAGATGATGAACATCAGTAGGGAAAATGCTTATGGTGTATTAGCTAAAGCAACCAGAGAGCTGATGACGAGAACTGTGGAAATCAGGAATCCTTTGGTTAAAGGCTTTGAGATTTTCCAGTGGACAAACTATGCCAAGTTCTCAAGCGAAAAATTAGAATTAGTTTTTAGTGAAGAGATATTGCCTTATCTTTTCCAGTTAAAAAAATTCATAAAATATAATCTGGAACATGTTAAGTCTTTTGAAAACAAATACTCTATGAGGATTTATGAGTGGTTATTAAAAGAACTAACACAAAAGAAAACTCACAAGGCAAATATAGAGATTAGCCTTGATGAATTTAAGTTCATGTTAATGCTTGAAAATAACTACCATGAGTTTAAAAGGCTTAACCAATGGGTTTTGAAACCAATAAGTAAAGATTTAAACACTTACAGCAATATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTTGATTTTCCAAGTTGAACTAGATAGACAAATGGATCTCGTAACCGAACTTGAGAACAACCAGATAAAAATGAATGGTGACAAAATACCAACAACCATTACATCAGATTCCTACCTACATAACGGACTAAGAAAAACACTACACGATGCTTTAACTGCAAAAATTCAGCTCACCAGTTTTGAGGCAAAATTTTTGAGTGACATGCAAAGTAAGTATGATCTCAATGGTTCGTTCTCATGGCTCACGCAAAAACAACGAACCACACTAGAGAACATACTGGCTAAATACGGAAGGATCTGA
Protein Sequence
MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI
rep_origin 1
Label Gene Product Position Strand
pSC101 ori 4467..4690 Fwd
DNA Sequence
GGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTC
terminator 3
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 2024..2096 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 2111..2139 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
lambda t0 terminator 5181..5276 Rev
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT