p406MET25

17421 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Nicolas Buchler, Fred Cross
Insert
MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
Mutation
MET25 promoter is between SacI and XbaI restricti…
Available Since
March 12, 2008
Availability
Available

Sequence Information

Length
657 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 638..657 Rev
DNA Sequence
TAATACGACTCACTATAGG
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 372..620 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC