pRha-ABE7.10-SpCas9-NG

170663 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells
Depositor
David Liu
Article
Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10
Insert
ABE7.10-SpCas9-NG
Tags
8xHIS and BP-NLS
Promoter
pRhaBAD
Available Since
Oct. 12, 2022
Availability
Available

Sequence Information

Length
7,707 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
8xHis — 8xHis affinity tag 381..405 Fwd
DNA Sequence
CACCATCACCATCATCATCATCAC
Protein Sequence
HHHHHHHH
SV40 NLS — nuclear localization signal of SV40 (si… 438..459 Fwd
DNA Sequence
CCGAAAAAAAAGCGTAAAGTT
Protein Sequence
PKKKRKV
SV40 NLS — nuclear localization signal of SV40 (si… 5808..5829 Fwd
DNA Sequence
CCGAAGAAGAAACGCAAAGTC
Protein Sequence
PKKKRKV
promoter 2
Label Gene Product Position Strand
cat promoter — — 34..125 Rev
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
rhaB promoter — — 228..347 Fwd
DNA Sequence
CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 6070..6616 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 4
Label Gene Product Position Strand
tonB terminator — — 133..164 Rev
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC
T7 terminator — — 5866..5914 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T3Te terminator — — 6011..6041 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
T7Te terminator — — 6880..6908 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG