Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

169353 Oryza sativa Yeast Expression

Plasmid Information

Purpose
Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
Depositor
Tony Carr
Insert
OsTIR1(F74A)
Mutation
F74A
Available Since
May 17, 2021
Availability
Available

Sequence Information

Length
8,216 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
AmpR bla beta-lactamase 527..1388 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
SV40 NLS — nuclear localization signal of SV40 (si… 6844..6865 Fwd
DNA Sequence
CCTAAAAAGAAGAGAAAGGTC
Protein Sequence
PKKKRKV
promoter 3
Label Gene Product Position Strand
AmpR promoter bla — 1388..1493 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ADH1 promoter S. cerevisiae ADH1 — 4311..5053 Fwd
DNA Sequence
GCCCTACAACAACTAAGAAAATGGCTATCATGCGGAAGCTGGTGAGAAGAACAGCATCGGGACAAGGGAAGGAAGAACAAAGACAAAGAAAACAAAAGAAAGCAATTGAAAACAAAACAAAACAATTTTCATTCCTTCTCTTATCATTCCTTTTCTTTTCTTTTCTCTCATTCAACGCACTCCATCGTATCCGTATTCCTCTTATTTTTTCTCTTTCTCTATATCCATTTCTTTCTCTCTAGGTGTGTCCTCTCTCTCTCTTCAATTTCTCTACTCCGCATTCCAACGCATCCTTCCCCCAACCTCCCATTTCCTCCTTACGGCCCGATAGCGATCGTCTTTCCCTCGCTATCACTCGCTACCGGCCCCTCCTCTGCACCGTAACCTCCTACGTATTTACCATATCATAAAGTTTTTTCCGACGCTTATCGCTGACCCCCTGTCGCCCTCCTATTGGCTTCCGGATTATCTTCTTGTCCATAAGGTGATCCATGCTTCCTGAAGATTCCCGAAATGTGTCCACTTTGGCGGGGAATCATTCCATCCACTTCTTTCTCTCTCGCTTTCCTCATTCGGCGCTCCCCTTCCGCGTCTCATTGGTCTTCCGCTCCGTTTTTGCTTTGCCGATGTTACTTGGGGAGAGGTGCGATAATCCTTTCGCAAAAACTCGGTTTGACGCCTCCCATGGTATAAATAGTGGGTGGTGGACAGGTGCCTTCGCTTTTCTTTAAGCAAGAGAATT
lac promoter — — 7630..7661 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 7606..7623 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 7675..7697 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 0..8216 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
ADH1 terminator S. cerevisiae ADH1 — 6886..7074 Fwd
DNA Sequence
GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG