Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

169352 Oryza sativa Yeast Expression

Plasmid Information

Purpose

Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

Depositor

Tony Carr

Insert

OsTIR1(WT)

Available Since

May 17, 2021

Availability

Available

Sequence Information

Length

8,216 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 3

Label	Gene	Product	Position	Strand
AmpR	bla	beta-lactamase	960..1821	Rev
DNA Sequence ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
OsTIR1	—	rice transport inhibitor response 1 pro…	5543..7268	Fwd
DNA Sequence ATGACTTATTTTCCGGAAGAGGTGGTCGAACACATTTTTTCTTTCTTGCCAGCGCAGCGAGACAGAAATACCGTTTCTTTGGTTTGTAAAGTTTGGTATGAAATCGAAAGACTGTCCAGGCGAGGCGTGTTTGTCGGTAACTGCTATGCGGTCAGAGCTGGAAGGGTGGCAGCGAGATTCCCGAATGTGAGGGCGTTGACTGTTAAGGGAAAACCACATTTTGCGGACTTTAACTTGGTTCCTCCTGACTGGGGAGGCTACGCCGGGCCCTGGATTGAGGCGGCAGCTCGAGGTTGTCATGGCTTGGAGGAGCTCCGCATGAAACGCATGGTGGTCTCCGACGAGTCTTTGGAGTTGCTCGCTCGGAGCTTCCCTAGGTTCCGGGCTTTGGTCCTGATATCATGTGAAGGATTTAGTACGGACGGATTGGCAGCGGTAGCCAGTCATTGCAAACTTCTGAGGGAGTTGGACTTGCAAGAGAACGAGGTGGAGGATCGAGGACCTCGCTGGCTGTCATGCTTTCCTGACAGTTGCACATCACTGGTTTCACTCAATTTCGCCTGTATTAAGGGAGAAGTGAATGCAGGTTCCCTCGAACGACTCGTTTCCCGGAGCCCCAATCTCCGATCCTTGAGACTCAACCGAAGTGTCAGTGTGGACACGCTTGCCAAGATCCTCTTGAGAACCCCTAATCTCGAGGATCTCGGAACCGGCAACCTTACTGATGATTTCCAAACAGAGAGCTATTTTAAACTCACCTCAGCGCTCGAAAAATGCAAAATGTTGAGGTCTTTGAGCGGGTTTTGGGATGCATCCCCAGTCTGTCTCAGCTTCATATATCCGTTGTGCGCTCAACTCACAGGGTTGAATCTTTCTTATGCACCTACCCTGGACGCTTCCGATTTGACCAAGATGATCTCTCGCTGCGTTAAGTTGCAACGCTTGTGGGTCCTGGATTGCATAAGTGACAAGGGGCTGCAAGTGGTGGCTTCCAGTTGTAAAGACCTCCAGGAACTGCGGGTATTTCCTAGCGATTTCTATGTAGCGGGATATAGCGCTGTCACTGAAGAAGGCCTTGTGGCAGTCTCTCTTGGATGCCCGAAGTTGAACTCATTGCTGTACTTCTGCCACCAAATGACAAACGCAGCTCTTGTTACCGTTGCTAAGAACTGTCCCAATTTCACGCGGTTCCGCCTTTGCATTTTGGAGCCTGGGAAGCCGGACGTGGTCACATCACAACCTTTGGACGAGGGGTTCGGCGCGATAGTGAGGGAATGTAAAGGCCTCCAAAGACTTAGTATCAGTGGTCTGCTTACCGACAAGGTCTTCATGTATATAGGAAAATACGCAAAGCAGCTCGAAATGTTGTCTATCGCATTCGCAGGTGATTCCGACAAGGGTATGATGCATGTAATGAATGGGTGTAAGAACCTCAGGAAACTGGAGATACGAGACTCACCTTTCGGTGACGCTGCCCTCCTTGGTAACTTTGCGCGGTATGAGACGATGCGGTCACTCTGGATGTCAAGTTGCAACGTTACGCTGAAAGGCTGTCAAGTTCTTGCATCAAAGATGCCAATGCTTAATGTTGAAGTTATAAATGAAAGAGACGGCAGCAATGAAATGGAGGAAAATCACGGGGACTTGCCCAAGGTTGAAAAATTGTATGTCTATAGAACCACGGCGGGTGCCAGAGATGACGCGCCCAATTTCGTGAAAATCCTT Protein Sequence MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL
SV40 NLS	—	nuclear localization signal of SV40 (si…	7277..7298	Fwd
DNA Sequence CCTAAAAAGAAGAGAAAGGTC Protein Sequence PKKKRKV

promoter 3

Label	Gene	Product	Position	Strand
AmpR promoter	bla	—	1821..1926	Rev
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ADH1 promoter	S. cerevisiae ADH1	—	4744..5486	Fwd
DNA Sequence GCCCTACAACAACTAAGAAAATGGCTATCATGCGGAAGCTGGTGAGAAGAACAGCATCGGGACAAGGGAAGGAAGAACAAAGACAAAGAAAACAAAAGAAAGCAATTGAAAACAAAACAAAACAATTTTCATTCCTTCTCTTATCATTCCTTTTCTTTTCTTTTCTCTCATTCAACGCACTCCATCGTATCCGTATTCCTCTTATTTTTTCTCTTTCTCTATATCCATTTCTTTCTCTCTAGGTGTGTCCTCTCTCTCTCTTCAATTTCTCTACTCCGCATTCCAACGCATCCTTCCCCCAACCTCCCATTTCCTCCTTACGGCCCGATAGCGATCGTCTTTCCCTCGCTATCACTCGCTACCGGCCCCTCCTCTGCACCGTAACCTCCTACGTATTTACCATATCATAAAGTTTTTTCCGACGCTTATCGCTGACCCCCTGTCGCCCTCCTATTGGCTTCCGGATTATCTTCTTGTCCATAAGGTGATCCATGCTTCCTGAAGATTCCCGAAATGTGTCCACTTTGGCGGGGAATCATTCCATCCACTTCTTTCTCTCTCGCTTTCCTCATTCGGCGCTCCCCTTCCGCGTCTCATTGGTCTTCCGCTCCGTTTTTGCTTTGCCGATGTTACTTGGGGAGAGGTGCGATAATCCTTTCGCAAAAACTCGGTTTGACGCCTCCCATGGTATAAATAGTGGGTGGTGGACAGGTGCCTTCGCTTTTCTTTAAGCAAGAGAATT
lac promoter	—	—	8063..8094	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	8039..8056	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	8108..8130	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	201..790	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

terminator 1

Label	Gene	Product	Position	Strand
ADH1 terminator	S. cerevisiae ADH1	—	7319..7507	Fwd
DNA Sequence GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG