| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| SmR | aadA | aminoglycoside adenylyltransferase (Mur… | 0..9334 | Fwd | |
|
DNA Sequence
ATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAA
Protein Sequence
MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK
|
|||||
| 6xHis | — | 6xHis affinity tag | 1716..1734 | Fwd | |
|
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
|
|||||
| LuxA | luxA | LuxA luciferase subunit | 2744..3824 | Fwd | |
|
DNA Sequence
ATGAAATTTGGAAACTTTTTGCTTACATACCAACCTCCCCAATTTTCCCAAACAGAGGTAATGAAACGTTTGGTTAAATTAGGTCGCATCTCTGAGGAGTGTGGTTTTGATACCGTATGGTTACTGGAGCATCATTTCACGGAGTTTGGTTTGCTTGGTAACCCTTATGTCGCTGCTGCATATTTACTTGGCGCGACTAAAAAATTGAATGTAGGAACTGCCGCTATTGTTCTTCCCACAGCCCATCCAGTACGCCAACTTGAAGATGTGAATTTATTGGATCAAATGTCAAAAGGACGATTTCGGTTTGGTATTTGCCGAGGGCTTTACAACAAGGACTTTCGCGTATTCGGCACAGATATGAATAACAGTCGCGCCTTAGCGGAATGCTGGTACGGGCTGATAAAGAATGGCATGACAGAGGGATATATGGAAGCTGATAATGAACATATCAAGTTCCATAAGGTAAAAGTAAACCCCGCGGCGTATAGCAGAGGTGGCGCACCGGTTTATGTGGTGGCTGAATCAGCTTCGACGACTGAGTGGGCTGCTCAATTTGGCCTACCGATGATATTAAGTTGGATTATAAATACTAACGAAAAGAAAGCACAACTTGAGCTTTATAATGAAGTGGCTCAAGAATATGGGCACGATATTCATAATATCGACCATTGCTTATCATATATAACATCTGTAGATCATGACTCAATTAAAGCGAAAGAGATTTGCCGGAAATTTCTGGGGCATTGGTATGATTCTTATGTGAATGCTACGACTATTTTTGATGATTCAGACCAAACAAGAGGTTATGATTTCAATAAAGGGCAGTGGCGTGACTTTGTATTAAAAGGACATAAAGATACTAATCGCCGTATTGATTACAGTTACGAAATCAATCCCGTGGGAACGCCGCAGGAATGTATTGACATAATTCAAAAAGACATTGATGCTACAGGAATATCAAATATTTGTTGTGGATTTGAAGCTAATGGAACAGTAGACGAAATTATTGCTTCCATGAAGCTCTTCCAGTCTGATGTCATGCCATTTCTTAAAGAAAAACAACGTTCGCTATTATAT
Protein Sequence
MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHHFTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFGICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAYSRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNIDHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFVLKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASMKLFQSDVMPFLKEKQRSLLY
|
|||||
| LuxB | luxB | LuxB luciferase subunit | 3887..4868 | Fwd | |
|
DNA Sequence
AAATTTGGATTGTTCTTCCTTAACTTCATCAATTCAACAACTGTTCAAGAACAGAGTATAGTTCGCATGCAGGAAATAACGGAGTATGTTGATAAGTTGAATTTTGAACAGATTTTAGTGTATGAAAATCATTTTTCAGATAATGGTGTTGTCGGCGCTCCTCTGACTGTTTCTGGTTTTCTGCTCGGTTTAACAGAGAAAATTAAAATTGGTTCATTAAATCACATCATTACAACTCATCATCCTGTCCGCATAGCGGAGGAAGCTTGCTTATTGGATCAGTTAAGTGAAGGGAGATTTATTTTAGGGTTTAGTGATTGCGAAAAAAAAGATGAAATGCATTTTTTTAATCGCCCGGTTGAATATCAACAGCAACTATTTGAAGAGTGTTATGAAATCATTAACGATGCTTTAACAACAGGCTATTGTAATCCAGATAACGATTTTTATAGCTTCCCTAAAATATCTGTAAATCCCCATGCTTATACGCCAGGCGGACCTCGGAAATATGTAACAGCAACCAGTCATCATATTGTTGAGTGGGCGGCCAAAAAAGGTATTCCTCTCATCTTTAAGTGGGATGATTCTAATGATGTTAGATATGAATATGCTGAAAGATATAAAGCCGTTGCGGATAAATATGACGTTGACCTATCAGAGATAGACCATCAGTTAATGATATTAGTTAACTATAACGAAGATAGTAATAAAGCTAAACAAGAGACGCGTGCATTTATTAGTGATTATGTTCTTGAAATGCACCCTAATGAAAATTTCGAAAATAAACTTGAAGAAATAATTGCAGAAAACGCTGTCGGAAATTATACGGAGTGTATAACTGCGGCTAAGTTGGCAATTGAAAAGTGTGGTGCGAAAAGTGTATTGCTGTCCTTTGAACCAATGAATGATTTGATGAGCCAAAAAAATGTAATCAATATTGTTGATGATAATATTAAGAAGTACCACACGGAATATACCTAA
Protein Sequence
KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGFSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYTPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEIDHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITAAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT
|
|||||
| T7 lysozyme | — | lysozyme from bacteriophage T7 | 5150..5603 | Fwd | |
|
DNA Sequence
ATGGCTCGTGTACAGTTTAAACAACGTGAATCTACTGACGCAATCTTTGTTCACTGCTCGGCTACCAAGCCAAGTCAGAATGTTGGTGTCCGTGAGATTCGCCAGTGGCACAAAGAGCAGGGTTGGCTCGATGTGGGATACCACTTTATCATCAAGCGAGACGGTACTGTGGAGGCAGGACGAGATGAGATGGCTGTAGGCTCTCACGCTAAGGGTTACAACCACAACTCTATCGGCGTCTGCCTTGTTGGTGGTATCGACGATAAAGGTAAGTTCGACGCTAACTTTACGCCAGCCCAAATGCAATCCCTTCGCTCACTGCTTGTCACACTGCTGGCTAAGTACGAAGGCGCTGTGCTTCGCGCCCATCATGAGGTGGCGCCGAAGGCTTCCCCTTCGTTCGACCTTAAGCGTTGGTGGGAGAAGAACGAACTGGTCACTTCTGACCGTGGT
Protein Sequence
MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDVGYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSLRSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 1450..1469 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| p15A ori | — | — | 281..827 | Rev | |
|
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||