pMSR:MSMEG_5277:Dendra

167416 M. smegmatis Bacterial Expression

Plasmid Information

Purpose
C-term fluorescent fusion
Depositor
Keith Derbyshire, Joseph Wade
Article
Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-
Insert
MSMEG_5277
Tags
Gly-Ala Linker/Dendra2/1x Flag Tag
Promoter
pmycTetO
Available Since
Feb. 25, 2022
Availability
Available

Sequence Information

Length
4,393 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 646..670 Rev
DNA Sequence
GACTACAAGGACGACGACGACAAG
Protein Sequence
DYKDDDDK
rep_origin 1
Label Gene Product Position Strand
ori — — 1688..2277 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAGGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA