pLSB-KAN

166700 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
Depositor
Robin Allshire
Article
Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
Promoter
adh15 promoter and tRNA Ser promoter
Available Since
April 28, 2021
Availability
Available

Sequence Information

Length
11,788 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 5
Label Gene Product Position Strand
AmpR bla beta-lactamase 962..1823 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 Cas9 (Csn1) endonuclease from the Strep… 2722..6826 Fwd
DNA Sequence
ATGGATAAGAAATACTCTATTGGCCTTGATATCGGAACTAACAGTGTAGGTTGGGCAGTCATAACAGATGAATACAAAGTTCCATCTAAGAAATTTAAAGTCCTTGGAAACACTGACCGACATAGTATAAAAAAGAATTTAATCGGCGCATTGCTTTTCGACTCTGGTGAAACGGCAGAAGCCACCCGATTGAAACGAACAGCAAGACGTCGATATACGCGAAGAAAGAACCGTATTTGCTATCTTCAGGAGATTTTTTCTAATGAAATGGCTAAGGTCGACGACTCCTTTTTCCACAGATTGGAGGAAAGTTTCCTTGTCGAAGAGGACAAAAAACACGAGCGTCACCCTATCTTCGGAAACATTGTAGACGAAGTGGCATATCATGAAAAGTATCCTACGATATACCATTTGCGTAAAAAGTTGGTCGACTCAACGGACAAGGCAGATTTGCGTTTGATATACTTGGCTCTTGCCCACATGATTAAATTTCGTGGCCATTTCTTAATTGAAGGTGACCTTAACCCAGATAACAGTGATGTGGACAAGTTGTTCATTCAATTGGTTCAGACCTACAATCAATTATTTGAGGAAAACCCAATCAATGCATCTGGCGTAGATGCAAAGGCTATTCTTTCCGCACGCCTTTCAAAATCACGCCGATTAGAGAATCTTATCGCTCAGTTACCTGGCGAGAAGAAAAATGGCTTGTTCGGTAATTTGATCGCTTTGAGTTTGGGCCTTACACCAAATTTTAAGTCCAACTTCGATCTTGCAGAAGATGCCAAATTACAATTAAGTAAGGATACGTACGACGATGATTTGGACAATTTACTTGCACAAATCGGTGACCAATATGCCGATCTTTTCTTGGCAGCTAAAAATTTATCAGACGCAATTTTATTGTCCGATATCCTTCGTGTGAACACCGAAATTACCAAGGCCCCACTTAGTGCTTCCATGATAAAACGCTATGATGAGCATCATCAGGACTTAACATTACTTAAAGCCTTGGTGCGCCAACAATTACCCGAAAAGTACAAAGAAATATTCTTCGACCAGTCCAAGAACGGTTATGCCGGCTACATCGATGGTGGTGCATCACAGGAGGAGTTCTACAAATTCATTAAACCAATCTTAGAAAAAATGGATGGTACCGAAGAGTTATTGGTAAAGTTAAATCGCGAAGACTTGCTTCGCAAACAACGAACATTCGACAACGGCTCCATCCCTCATCAAATTCACCTTGGCGAATTACATGCCATCTTACGCAGACAAGAAGACTTTTATCCATTCTTGAAGGATAACCGCGAGAAAATTGAGAAGATCCTTACCTTTAGAATTCCATACTACGTAGGCCCTTTGGCTAGAGGCAACTCCAGATTTGCATGGATGACCAGAAAATCTGAGGAAACCATCACGCCCTGGAACTTCGAAGAAGTCGTAGATAAGGGCGCAAGTGCACAGTCCTTTATTGAAAGAATGACCAATTTTGATAAGAACTTACCTAACGAAAAGGTCTTACCAAAGCATAGTTTACTTTATGAATACTTCACGGTGTACAACGAATTAACAAAAGTCAAATACGTTACTGAGGGAATGAGAAAGCCTGCATTCTTGTCCGGAGAACAAAAGAAGGCCATAGTTGACCTTCTTTTCAAAACGAATCGAAAGGTCACGGTAAAACAACTTAAGGAGGATTACTTTAAGAAGATAGAATGCTTTGATAGTGTTGAGATATCAGGTGTTGAAGACCGATTCAATGCATCATTGGGAACGTACCACGACCTTTTGAAGATCATAAAAGATAAAGATTTTTTAGACAATGAGGAGAACGAAGACATTTTAGAAGACATCGTTTTGACACTTACGTTATTTGAGGACAGAGAGATGATCGAGGAGCGTCTTAAAACGTATGCACATTTATTCGACGACAAAGTGATGAAACAACTTAAGCGCAGAAGATATACCGGTTGGGGAAGACTTTCTCGAAAGTTGATCAACGGCATCCGCGATAAGCAAAGTGGAAAAACCATTTTGGATTTCTTAAAGTCTGATGGCTTTGCTAATCGCAACTTCATGCAATTGATTCATGACGATTCATTAACTTTCAAGGAAGACATACAGAAGGCCCAAGTTTCAGGTCAGGGAGATAGTTTGCACGAGCACATCGCTAATTTAGCTGGTTCCCCTGCTATAAAAAAGGGTATACTTCAAACCGTAAAAGTCGTAGATGAGTTGGTAAAAGTCATGGGCAGACATAAACCAGAAAATATAGTAATTGAGATGGCCCGAGAAAATCAAACGACGCAAAAGGGCCAAAAAAACTCTAGAGAAAGAATGAAGCGTATCGAGGAGGGCATAAAGGAGTTGGGTTCTCAGATATTGAAGGAGCACCCCGTTGAGAACACTCAGCTTCAGAATGAGAAGCTTTATTTATATTATTTACAAAACGGTCGTGATATGTATGTGGATCAGGAGTTGGACATAAACAGATTGTCAGATTACGATGTGGATCATATCGTCCCCCAATCATTCTTAAAGGACGATTCAATTGACAACAAAGTTCTTACGCGTAGTGACAAGAACAGAGGCAAATCAGACAATGTACCTAGTGAGGAAGTAGTGAAAAAGATGAAGAACTATTGGAGACAACTTCTTAATGCTAAGTTAATAACGCAGCGAAAGTTTGATAACTTAACTAAAGCCGAACGTGGCGGACTTTCAGAGTTGGATAAAGCAGGCTTTATCAAACGTCAATTGGTAGAGACTCGTCAGATCACTAAGCATGTGGCCCAAATACTTGATTCACGCATGAATACTAAGTACGATGAAAACGATAAATTGATTCGTGAGGTTAAGGTAATCACGTTGAAATCTAAGCTTGTTTCCGACTTTCGTAAGGATTTTCAATTCTACAAGGTTCGAGAGATTAATAATTACCACCATGCACATGACGCATACTTGAACGCAGTCGTAGGTACAGCATTGATCAAGAAGTACCCAAAATTAGAGTCAGAATTTGTCTACGGTGACTACAAAGTATATGATGTCCGAAAGATGATAGCCAAGTCTGAGCAAGAGATAGGAAAGGCCACAGCTAAGTATTTTTTTTACTCCAACATTATGAATTTCTTTAAGACAGAGATTACTTTAGCTAACGGTGAAATACGAAAAAGACCCCTTATCGAAACGAATGGAGAGACAGGTGAGATTGTTTGGGACAAAGGTCGAGATTTCGCCACTGTGAGAAAAGTCTTGAGTATGCCACAGGTCAATATTGTTAAGAAAACAGAAGTACAGACTGGTGGATTTTCTAAAGAATCCATTTTGCCCAAGAGAAATTCCGACAAACTTATTGCACGCAAGAAGGATTGGGATCCCAAGAAATACGGCGGTTTCGACAGTCCCACTGTAGCATACTCAGTGTTGGTCGTGGCTAAGGTGGAAAAGGGTAAGTCTAAGAAGCTTAAATCTGTGAAGGAATTGCTTGGTATCACGATCATGGAGCGCTCATCTTTTGAGAAAAATCCTATAGACTTTCTTGAAGCTAAAGGCTATAAAGAGGTGAAAAAGGACCTTATAATTAAGTTACCAAAGTACTCTCTTTTCGAACTTGAGAACGGACGCAAGAGAATGCTTGCCAGTGCTGGAGAATTACAGAAGGGTAATGAGTTAGCATTGCCATCAAAGTACGTAAACTTCTTATATTTAGCCTCCCACTATGAGAAATTAAAGGGTTCACCCGAGGACAATGAACAAAAGCAATTATTCGTAGAACAACATAAACACTATTTGGACGAAATTATAGAGCAAATCTCTGAATTTTCCAAAAGAGTGATTTTAGCTGACGCAAATCTTGATAAGGTTTTATCTGCCTATAACAAGCATCGCGACAAACCCATCAGAGAACAAGCCGAAAATATCATTCACTTATTCACACTTACGAATCTTGGAGCCCCTGCCGCTTTCAAGTATTTTGATACTACCATAGACCGTAAACGCTATACGTCTACTAAGGAAGTCTTAGACGCCACTCTTATTCACCAAAGTATAACCGGCTTGTATGAGACAAGAATTGATTTGTCTCAATTGGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS nuclear localization signal of SV40 (si… 6838..6859 Fwd
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
KanR aph(3')-Ia aminoglycoside phosphotransferase 8937..9747 Fwd
DNA Sequence
ATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
superfolder GFP GFP variant that folds robustly even wh… 10611..11325 Fwd
DNA Sequence
ATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTTCCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAGGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAA
Protein Sequence
MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
promoter 4
Label Gene Product Position Strand
AmpR promoter bla 1823..1928 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ADH1 promoter S. cerevisiae ADH1 1960..2702 Fwd
DNA Sequence
GCCCTACAACAACTAAGAAAATGGCTATCATGCGGAAGCTGGTGAGAAGAACAGCATCGGGACAAGGGAAGGAAGAACAAAGACAAAGAAAACAAAAGAAAGCAATTGAAAACAAAACAAAACAATTTTCATTCCTTCTCTTATCATTCCTTTTCTTTTCTTTTCTCTCATTCAACGCACTCCATCGTATCCGTATTCCTCTTATTTTTTCTCTTTCTCTATATCCATTTCTTTCTCTCTAGGTGTGTCCTCTCTCTCTCTTCAATTTCTCTACTCCGCATTCCAACGCATCCTTCCCCCAACCTCCCATTTCCTCCTTACGGCCCGATAGCGATCGTCTTTCCCTCGCTATCACTCGCTACCGGCCCCTCCTCTGCACCGTAACCTCCTACGTATTTACCATATCATAAAGTTTTTTCCGACGCTTATCGCTGACCCCCTGTCGCCCTCCTATTGGCTTCCGGATTATCTTCTTGTCCATAAGGTGATCCATGCTTCCTGAAGATTCCCGAAATGTGTCCACTTTGGCGGGGAATCATTCCATCCACTTCTTTCTCTCTCGCTTTCCTCATTCGGCGCTCCCCTTCCGCGTCTCATTGGTCTTCCGCTCCGTTTTTGCTTTGCCGATGTTACTTGGGGAGAGGTGCGATAATCCTTTCGCAAAAACTCGGTTTGACGCCTCCCATGGTAAATATAGTGGGTGGTGGACAGGTGCCTTCGCTTTTCTTTAAGCAAGAGAATT
TEF promoter 8593..8937 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
lac promoter 11637..11668 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 11613..11630 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 11682..11704 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
ori 203..792 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
ARS1 7738..8520 Fwd
DNA Sequence
TCTATAATTATAGCTAAAAATTGTATTTTAATTTGTATTTTTTGTAATTTTATTTCTCATTGTTTTACTTAAAATGAATGCGAATTAGAGAAAACTTCAACGAAATGTCAAAATAAGCTCAGCAAAATATACAATTTTAGGGAAAGCGATCAGCAACTTATTCTCCGTTGTAGATTCTTTTTCTTCATAAATTCCAACAATGTAACTGCCAATTGATTTTTCTCTAGATGCTAATAAATTGAAACTAATGAATATAATAACACAGGTTTTGATAAATTGTAAACTCTTACTCAAAAAAAGAATGTTAAAACTAATTTAACCTTTTTATAAAGGCTCATGGCGTTAAAGAGGATAGTAAAAATTCTACAATTTTTTAATTTAATGTCACAATTGTCTTGCGAATATAATTCTATTAATTTTCGCTAACTGCTGTTTTAGTACTATTACTTTCAATATGCCTTATATGCAACTTTAATTCATGAAGAATGCAATATTTTTGCAATCTTTCTTGTACTGAGCGCTTTTTATCATTTAGTACTCATTATTTAATTTTTCAGTAAAGGGCAATAAATAAATTTGTAGAAGATGCAATGTAATCTCCTCTATCCTTTTGCTCATATGTTTATGAGTATACCTAGTCTAGAAAGGCTTGTATTTAAAATATTATTCAATAAAAATTCACAATTTTTACGACATGTGCTACCATTCACTTAACTTCCTGATTATAAAATTGGTTCGTTTATACTAATTACTTAAGTACCTTTAACTAAACAAAATGCCTA
terminator 5
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 6876..7124 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
TEF terminator 9752..9950 Fwd
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG
rrnB T1 terminator Escherichia coli rrnB 11336..11408 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 11423..11451 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
SUP4 terminator 11549..11569 Fwd
DNA Sequence
TTTTTTTGTTTTTTATGTCT