pLSB-HYG

166699 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
Depositor
Robin Allshire
Article
Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
Promoter
adh15 promoter and tRNA promoter
Available Since
April 28, 2021
Availability
Available

Sequence Information

Length
12,007 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 5
Label Gene Product Position Strand
superfolder GFP GFP variant that folds robustly even wh… 661..1375 Fwd
DNA Sequence
ATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTTCCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAGGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAA
Protein Sequence
MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
AmpR bla beta-lactamase 2800..3661 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 Cas9 (Csn1) endonuclease from the Strep… 4560..8664 Fwd
DNA Sequence
ATGGATAAGAAATACTCTATTGGCCTTGATATCGGAACTAACAGTGTAGGTTGGGCAGTCATAACAGATGAATACAAAGTTCCATCTAAGAAATTTAAAGTCCTTGGAAACACTGACCGACATAGTATAAAAAAGAATTTAATCGGCGCATTGCTTTTCGACTCTGGTGAAACGGCAGAAGCCACCCGATTGAAACGAACAGCAAGACGTCGATATACGCGAAGAAAGAACCGTATTTGCTATCTTCAGGAGATTTTTTCTAATGAAATGGCTAAGGTCGACGACTCCTTTTTCCACAGATTGGAGGAAAGTTTCCTTGTCGAAGAGGACAAAAAACACGAGCGTCACCCTATCTTCGGAAACATTGTAGACGAAGTGGCATATCATGAAAAGTATCCTACGATATACCATTTGCGTAAAAAGTTGGTCGACTCAACGGACAAGGCAGATTTGCGTTTGATATACTTGGCTCTTGCCCACATGATTAAATTTCGTGGCCATTTCTTAATTGAAGGTGACCTTAACCCAGATAACAGTGATGTGGACAAGTTGTTCATTCAATTGGTTCAGACCTACAATCAATTATTTGAGGAAAACCCAATCAATGCATCTGGCGTAGATGCAAAGGCTATTCTTTCCGCACGCCTTTCAAAATCACGCCGATTAGAGAATCTTATCGCTCAGTTACCTGGCGAGAAGAAAAATGGCTTGTTCGGTAATTTGATCGCTTTGAGTTTGGGCCTTACACCAAATTTTAAGTCCAACTTCGATCTTGCAGAAGATGCCAAATTACAATTAAGTAAGGATACGTACGACGATGATTTGGACAATTTACTTGCACAAATCGGTGACCAATATGCCGATCTTTTCTTGGCAGCTAAAAATTTATCAGACGCAATTTTATTGTCCGATATCCTTCGTGTGAACACCGAAATTACCAAGGCCCCACTTAGTGCTTCCATGATAAAACGCTATGATGAGCATCATCAGGACTTAACATTACTTAAAGCCTTGGTGCGCCAACAATTACCCGAAAAGTACAAAGAAATATTCTTCGACCAGTCCAAGAACGGTTATGCCGGCTACATCGATGGTGGTGCATCACAGGAGGAGTTCTACAAATTCATTAAACCAATCTTAGAAAAAATGGATGGTACCGAAGAGTTATTGGTAAAGTTAAATCGCGAAGACTTGCTTCGCAAACAACGAACATTCGACAACGGCTCCATCCCTCATCAAATTCACCTTGGCGAATTACATGCCATCTTACGCAGACAAGAAGACTTTTATCCATTCTTGAAGGATAACCGCGAGAAAATTGAGAAGATCCTTACCTTTAGAATTCCATACTACGTAGGCCCTTTGGCTAGAGGCAACTCCAGATTTGCATGGATGACCAGAAAATCTGAGGAAACCATCACGCCCTGGAACTTCGAAGAAGTCGTAGATAAGGGCGCAAGTGCACAGTCCTTTATTGAAAGAATGACCAATTTTGATAAGAACTTACCTAACGAAAAGGTCTTACCAAAGCATAGTTTACTTTATGAATACTTCACGGTGTACAACGAATTAACAAAAGTCAAATACGTTACTGAGGGAATGAGAAAGCCTGCATTCTTGTCCGGAGAACAAAAGAAGGCCATAGTTGACCTTCTTTTCAAAACGAATCGAAAGGTCACGGTAAAACAACTTAAGGAGGATTACTTTAAGAAGATAGAATGCTTTGATAGTGTTGAGATATCAGGTGTTGAAGACCGATTCAATGCATCATTGGGAACGTACCACGACCTTTTGAAGATCATAAAAGATAAAGATTTTTTAGACAATGAGGAGAACGAAGACATTTTAGAAGACATCGTTTTGACACTTACGTTATTTGAGGACAGAGAGATGATCGAGGAGCGTCTTAAAACGTATGCACATTTATTCGACGACAAAGTGATGAAACAACTTAAGCGCAGAAGATATACCGGTTGGGGAAGACTTTCTCGAAAGTTGATCAACGGCATCCGCGATAAGCAAAGTGGAAAAACCATTTTGGATTTCTTAAAGTCTGATGGCTTTGCTAATCGCAACTTCATGCAATTGATTCATGACGATTCATTAACTTTCAAGGAAGACATACAGAAGGCCCAAGTTTCAGGTCAGGGAGATAGTTTGCACGAGCACATCGCTAATTTAGCTGGTTCCCCTGCTATAAAAAAGGGTATACTTCAAACCGTAAAAGTCGTAGATGAGTTGGTAAAAGTCATGGGCAGACATAAACCAGAAAATATAGTAATTGAGATGGCCCGAGAAAATCAAACGACGCAAAAGGGCCAAAAAAACTCTAGAGAAAGAATGAAGCGTATCGAGGAGGGCATAAAGGAGTTGGGTTCTCAGATATTGAAGGAGCACCCCGTTGAGAACACTCAGCTTCAGAATGAGAAGCTTTATTTATATTATTTACAAAACGGTCGTGATATGTATGTGGATCAGGAGTTGGACATAAACAGATTGTCAGATTACGATGTGGATCATATCGTCCCCCAATCATTCTTAAAGGACGATTCAATTGACAACAAAGTTCTTACGCGTAGTGACAAGAACAGAGGCAAATCAGACAATGTACCTAGTGAGGAAGTAGTGAAAAAGATGAAGAACTATTGGAGACAACTTCTTAATGCTAAGTTAATAACGCAGCGAAAGTTTGATAACTTAACTAAAGCCGAACGTGGCGGACTTTCAGAGTTGGATAAAGCAGGCTTTATCAAACGTCAATTGGTAGAGACTCGTCAGATCACTAAGCATGTGGCCCAAATACTTGATTCACGCATGAATACTAAGTACGATGAAAACGATAAATTGATTCGTGAGGTTAAGGTAATCACGTTGAAATCTAAGCTTGTTTCCGACTTTCGTAAGGATTTTCAATTCTACAAGGTTCGAGAGATTAATAATTACCACCATGCACATGACGCATACTTGAACGCAGTCGTAGGTACAGCATTGATCAAGAAGTACCCAAAATTAGAGTCAGAATTTGTCTACGGTGACTACAAAGTATATGATGTCCGAAAGATGATAGCCAAGTCTGAGCAAGAGATAGGAAAGGCCACAGCTAAGTATTTTTTTTACTCCAACATTATGAATTTCTTTAAGACAGAGATTACTTTAGCTAACGGTGAAATACGAAAAAGACCCCTTATCGAAACGAATGGAGAGACAGGTGAGATTGTTTGGGACAAAGGTCGAGATTTCGCCACTGTGAGAAAAGTCTTGAGTATGCCACAGGTCAATATTGTTAAGAAAACAGAAGTACAGACTGGTGGATTTTCTAAAGAATCCATTTTGCCCAAGAGAAATTCCGACAAACTTATTGCACGCAAGAAGGATTGGGATCCCAAGAAATACGGCGGTTTCGACAGTCCCACTGTAGCATACTCAGTGTTGGTCGTGGCTAAGGTGGAAAAGGGTAAGTCTAAGAAGCTTAAATCTGTGAAGGAATTGCTTGGTATCACGATCATGGAGCGCTCATCTTTTGAGAAAAATCCTATAGACTTTCTTGAAGCTAAAGGCTATAAAGAGGTGAAAAAGGACCTTATAATTAAGTTACCAAAGTACTCTCTTTTCGAACTTGAGAACGGACGCAAGAGAATGCTTGCCAGTGCTGGAGAATTACAGAAGGGTAATGAGTTAGCATTGCCATCAAAGTACGTAAACTTCTTATATTTAGCCTCCCACTATGAGAAATTAAAGGGTTCACCCGAGGACAATGAACAAAAGCAATTATTCGTAGAACAACATAAACACTATTTGGACGAAATTATAGAGCAAATCTCTGAATTTTCCAAAAGAGTGATTTTAGCTGACGCAAATCTTGATAAGGTTTTATCTGCCTATAACAAGCATCGCGACAAACCCATCAGAGAACAAGCCGAAAATATCATTCACTTATTCACACTTACGAATCTTGGAGCCCCTGCCGCTTTCAAGTATTTTGATACTACCATAGACCGTAAACGCTATACGTCTACTAAGGAAGTCTTAGACGCCACTCTTATTCACCAAAGTATAACCGGCTTGTATGAGACAAGAATTGATTTGTCTCAATTGGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS nuclear localization signal of SV40 (si… 8676..8697 Fwd
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 10775..11804 Fwd
DNA Sequence
ATGGGTAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCGGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCGGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAA
Protein Sequence
MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
promoter 4
Label Gene Product Position Strand
lac promoter 1687..1718 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 3661..3766 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ADH1 promoter S. cerevisiae ADH1 3798..4540 Fwd
DNA Sequence
GCCCTACAACAACTAAGAAAATGGCTATCATGCGGAAGCTGGTGAGAAGAACAGCATCGGGACAAGGGAAGGAAGAACAAAGACAAAGAAAACAAAAGAAAGCAATTGAAAACAAAACAAAACAATTTTCATTCCTTCTCTTATCATTCCTTTTCTTTTCTTTTCTCTCATTCAACGCACTCCATCGTATCCGTATTCCTCTTATTTTTTCTCTTTCTCTATATCCATTTCTTTCTCTCTAGGTGTGTCCTCTCTCTCTCTTCAATTTCTCTACTCCGCATTCCAACGCATCCTTCCCCCAACCTCCCATTTCCTCCTTACGGCCCGATAGCGATCGTCTTTCCCTCGCTATCACTCGCTACCGGCCCCTCCTCTGCACCGTAACCTCCTACGTATTTACCATATCATAAAGTTTTTTCCGACGCTTATCGCTGACCCCCTGTCGCCCTCCTATTGGCTTCCGGATTATCTTCTTGTCCATAAGGTGATCCATGCTTCCTGAAGATTCCCGAAATGTGTCCACTTTGGCGGGGAATCATTCCATCCACTTCTTTCTCTCTCGCTTTCCTCATTCGGCGCTCCCCTTCCGCGTCTCATTGGTCTTCCGCTCCGTTTTTGCTTTGCCGATGTTACTTGGGGAGAGGTGCGATAATCCTTTCGCAAAAACTCGGTTTGACGCCTCCCATGGTAAATATAGTGGGTGGTGGACAGGTGCCTTCGCTTTTCTTTAAGCAAGAGAATT
TEF promoter 10431..10775 Fwd
DNA Sequence
GAAATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCGGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
protein_bind 2
Label Gene Product Position Strand
lac operator 1663..1680 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 1732..1754 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
ori 2041..2630 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
ARS1 9576..10358 Fwd
DNA Sequence
TCTATAATTATAGCTAAAAATTGTATTTTAATTTGTATTTTTTGTAATTTTATTTCTCATTGTTTTACTTAAAATGAATGCGAATTAGAGAAAACTTCAACGAAATGTCAAAATAAGCTCAGCAAAATATACAATTTTAGGGAAAGCGATCAGCAACTTATTCTCCGTTGTAGATTCTTTTTCTTCATAAATTCCAACAATGTAACTGCCAATTGATTTTTCTCTAGATGCTAATAAATTGAAACTAATGAATATAATAACACAGGTTTTGATAAATTGTAAACTCTTACTCAAAAAAAGAATGTTAAAACTAATTTAACCTTTTTATAAAGGCTCATGGCGTTAAAGAGGATAGTAAAAATTCTACAATTTTTTAATTTAATGTCACAATTGTCTTGCGAATATAATTCTATTAATTTTCGCTAACTGCTGTTTTAGTACTATTACTTTCAATATGCCTTATATGCAACTTTAATTCATGAAGAATGCAATATTTTTGCAATCTTTCTTGTACTGAGCGCTTTTTATCATTTAGTACTCATTATTTAATTTTTCAGTAAAGGGCAATAAATAAATTTGTAGAAGATGCAATGTAATCTCCTCTATCCTTTTGCTCATATGTTTATGAGTATACCTAGTCTAGAAAGGCTTGTATTTAAAATATTATTCAATAAAAATTCACAATTTTTACGACATGTGCTACCATTCACTTAACTTCCTGATTATAAAATTGGTTCGTTTATACTAATTACTTAAGTACCTTTAACTAAACAAAATGCCTA
terminator 5
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 1386..1458 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 1473..1501 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
SUP4 terminator 1599..1619 Fwd
DNA Sequence
TTTTTTTGTTTTTTATGTCT
CYC1 terminator S. cerevisiae CYC1 8714..8962 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
TEF terminator 11809..12007 Fwd
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG