pLSB-NAT

166698 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
Depositor
Robin Allshire
Article
Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
Promoter
adh15 promoter and tRNA promoter
Available Since
April 28, 2021
Availability
Available

Sequence Information

Length
11,551 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
AmpR bla beta-lactamase 1073..1934 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 2833..6937 Fwd
DNA Sequence
ATGGATAAGAAATACTCTATTGGCCTTGATATCGGAACTAACAGTGTAGGTTGGGCAGTCATAACAGATGAATACAAAGTTCCATCTAAGAAATTTAAAGTCCTTGGAAACACTGACCGACATAGTATAAAAAAGAATTTAATCGGCGCATTGCTTTTCGACTCTGGTGAAACGGCAGAAGCCACCCGATTGAAACGAACAGCAAGACGTCGATATACGCGAAGAAAGAACCGTATTTGCTATCTTCAGGAGATTTTTTCTAATGAAATGGCTAAGGTCGACGACTCCTTTTTCCACAGATTGGAGGAAAGTTTCCTTGTCGAAGAGGACAAAAAACACGAGCGTCACCCTATCTTCGGAAACATTGTAGACGAAGTGGCATATCATGAAAAGTATCCTACGATATACCATTTGCGTAAAAAGTTGGTCGACTCAACGGACAAGGCAGATTTGCGTTTGATATACTTGGCTCTTGCCCACATGATTAAATTTCGTGGCCATTTCTTAATTGAAGGTGACCTTAACCCAGATAACAGTGATGTGGACAAGTTGTTCATTCAATTGGTTCAGACCTACAATCAATTATTTGAGGAAAACCCAATCAATGCATCTGGCGTAGATGCAAAGGCTATTCTTTCCGCACGCCTTTCAAAATCACGCCGATTAGAGAATCTTATCGCTCAGTTACCTGGCGAGAAGAAAAATGGCTTGTTCGGTAATTTGATCGCTTTGAGTTTGGGCCTTACACCAAATTTTAAGTCCAACTTCGATCTTGCAGAAGATGCCAAATTACAATTAAGTAAGGATACGTACGACGATGATTTGGACAATTTACTTGCACAAATCGGTGACCAATATGCCGATCTTTTCTTGGCAGCTAAAAATTTATCAGACGCAATTTTATTGTCCGATATCCTTCGTGTGAACACCGAAATTACCAAGGCCCCACTTAGTGCTTCCATGATAAAACGCTATGATGAGCATCATCAGGACTTAACATTACTTAAAGCCTTGGTGCGCCAACAATTACCCGAAAAGTACAAAGAAATATTCTTCGACCAGTCCAAGAACGGTTATGCCGGCTACATCGATGGTGGTGCATCACAGGAGGAGTTCTACAAATTCATTAAACCAATCTTAGAAAAAATGGATGGTACCGAAGAGTTATTGGTAAAGTTAAATCGCGAAGACTTGCTTCGCAAACAACGAACATTCGACAACGGCTCCATCCCTCATCAAATTCACCTTGGCGAATTACATGCCATCTTACGCAGACAAGAAGACTTTTATCCATTCTTGAAGGATAACCGCGAGAAAATTGAGAAGATCCTTACCTTTAGAATTCCATACTACGTAGGCCCTTTGGCTAGAGGCAACTCCAGATTTGCATGGATGACCAGAAAATCTGAGGAAACCATCACGCCCTGGAACTTCGAAGAAGTCGTAGATAAGGGCGCAAGTGCACAGTCCTTTATTGAAAGAATGACCAATTTTGATAAGAACTTACCTAACGAAAAGGTCTTACCAAAGCATAGTTTACTTTATGAATACTTCACGGTGTACAACGAATTAACAAAAGTCAAATACGTTACTGAGGGAATGAGAAAGCCTGCATTCTTGTCCGGAGAACAAAAGAAGGCCATAGTTGACCTTCTTTTCAAAACGAATCGAAAGGTCACGGTAAAACAACTTAAGGAGGATTACTTTAAGAAGATAGAATGCTTTGATAGTGTTGAGATATCAGGTGTTGAAGACCGATTCAATGCATCATTGGGAACGTACCACGACCTTTTGAAGATCATAAAAGATAAAGATTTTTTAGACAATGAGGAGAACGAAGACATTTTAGAAGACATCGTTTTGACACTTACGTTATTTGAGGACAGAGAGATGATCGAGGAGCGTCTTAAAACGTATGCACATTTATTCGACGACAAAGTGATGAAACAACTTAAGCGCAGAAGATATACCGGTTGGGGAAGACTTTCTCGAAAGTTGATCAACGGCATCCGCGATAAGCAAAGTGGAAAAACCATTTTGGATTTCTTAAAGTCTGATGGCTTTGCTAATCGCAACTTCATGCAATTGATTCATGACGATTCATTAACTTTCAAGGAAGACATACAGAAGGCCCAAGTTTCAGGTCAGGGAGATAGTTTGCACGAGCACATCGCTAATTTAGCTGGTTCCCCTGCTATAAAAAAGGGTATACTTCAAACCGTAAAAGTCGTAGATGAGTTGGTAAAAGTCATGGGCAGACATAAACCAGAAAATATAGTAATTGAGATGGCCCGAGAAAATCAAACGACGCAAAAGGGCCAAAAAAACTCTAGAGAAAGAATGAAGCGTATCGAGGAGGGCATAAAGGAGTTGGGTTCTCAGATATTGAAGGAGCACCCCGTTGAGAACACTCAGCTTCAGAATGAGAAGCTTTATTTATATTATTTACAAAACGGTCGTGATATGTATGTGGATCAGGAGTTGGACATAAACAGATTGTCAGATTACGATGTGGATCATATCGTCCCCCAATCATTCTTAAAGGACGATTCAATTGACAACAAAGTTCTTACGCGTAGTGACAAGAACAGAGGCAAATCAGACAATGTACCTAGTGAGGAAGTAGTGAAAAAGATGAAGAACTATTGGAGACAACTTCTTAATGCTAAGTTAATAACGCAGCGAAAGTTTGATAACTTAACTAAAGCCGAACGTGGCGGACTTTCAGAGTTGGATAAAGCAGGCTTTATCAAACGTCAATTGGTAGAGACTCGTCAGATCACTAAGCATGTGGCCCAAATACTTGATTCACGCATGAATACTAAGTACGATGAAAACGATAAATTGATTCGTGAGGTTAAGGTAATCACGTTGAAATCTAAGCTTGTTTCCGACTTTCGTAAGGATTTTCAATTCTACAAGGTTCGAGAGATTAATAATTACCACCATGCACATGACGCATACTTGAACGCAGTCGTAGGTACAGCATTGATCAAGAAGTACCCAAAATTAGAGTCAGAATTTGTCTACGGTGACTACAAAGTATATGATGTCCGAAAGATGATAGCCAAGTCTGAGCAAGAGATAGGAAAGGCCACAGCTAAGTATTTTTTTTACTCCAACATTATGAATTTCTTTAAGACAGAGATTACTTTAGCTAACGGTGAAATACGAAAAAGACCCCTTATCGAAACGAATGGAGAGACAGGTGAGATTGTTTGGGACAAAGGTCGAGATTTCGCCACTGTGAGAAAAGTCTTGAGTATGCCACAGGTCAATATTGTTAAGAAAACAGAAGTACAGACTGGTGGATTTTCTAAAGAATCCATTTTGCCCAAGAGAAATTCCGACAAACTTATTGCACGCAAGAAGGATTGGGATCCCAAGAAATACGGCGGTTTCGACAGTCCCACTGTAGCATACTCAGTGTTGGTCGTGGCTAAGGTGGAAAAGGGTAAGTCTAAGAAGCTTAAATCTGTGAAGGAATTGCTTGGTATCACGATCATGGAGCGCTCATCTTTTGAGAAAAATCCTATAGACTTTCTTGAAGCTAAAGGCTATAAAGAGGTGAAAAAGGACCTTATAATTAAGTTACCAAAGTACTCTCTTTTCGAACTTGAGAACGGACGCAAGAGAATGCTTGCCAGTGCTGGAGAATTACAGAAGGGTAATGAGTTAGCATTGCCATCAAAGTACGTAAACTTCTTATATTTAGCCTCCCACTATGAGAAATTAAAGGGTTCACCCGAGGACAATGAACAAAAGCAATTATTCGTAGAACAACATAAACACTATTTGGACGAAATTATAGAGCAAATCTCTGAATTTTCCAAAAGAGTGATTTTAGCTGACGCAAATCTTGATAAGGTTTTATCTGCCTATAACAAGCATCGCGACAAACCCATCAGAGAACAAGCCGAAAATATCATTCACTTATTCACACTTACGAATCTTGGAGCCCCTGCCGCTTTCAAGTATTTTGATACTACCATAGACCGTAAACGCTATACGTCTACTAAGGAAGTCTTAGACGCCACTCTTATTCACCAAAGTATAACCGGCTTGTATGAGACAAGAATTGATTTGTCTCAATTGGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS — nuclear localization signal of SV40 (si… 6949..6970 Fwd
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
NrsR Streptomyces noursei nat1 nourseothricin acetyltransferase 9048..9621 Fwd
DNA Sequence
ATGGGTACCACTCTTGACGACACGGCTTACCGGTACCGCACCAGTGTCCCGGGGGACGCCGAGGCCATCGAGGCACTGGATGGGTCCTTCACCACCGACACCGTCTTCCGCGTCACCGCCACCGGGGACGGCTTCACCCTGCGGGAGGTGCCGGTGGACCCGCCCCTGACCAAGGTGTTCCCCGACGACGAATCGGACGACGAATCGGACGACGGGGAGGACGGCGACCCGGACTCCCGGACGTTCGTCGCGTACGGGGACGACGGCGACCTGGCGGGCTTCGTGGTCGTCTCGTACTCCGGCTGGAACCGCCGGCTGACCGTCGAGGACATCGAGGTCGCCCCGGAGCACCGGGGGCACGGGGTCGGGCGCGCGTTGATGGGGCTCGCGACGGAGTTCGCCCGCGAGCGGGGCGCCGGGCACCTCTGGCTGGAGGTCACCAACGTCAACGCACCGGCGATCCACGCGTACCGGCGGATGGGGTTCACCCTCTGCGGCCTGGACACCGCCCTGTACGACGGCACCGCCTCGGACGGCGAGCAGGCGCTCTACATGAGCATGCCCTGCCCCTAA
Protein Sequence
MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCGLDTALYDGTASDGEQALYMSMPCP
superfolder GFP — GFP variant that folds robustly even wh… 10485..11199 Fwd
DNA Sequence
ATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTTCCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAGGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAA
Protein Sequence
MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
promoter 4
Label Gene Product Position Strand
AmpR promoter bla — 1934..2039 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ADH1 promoter S. cerevisiae ADH1 — 2071..2813 Fwd
DNA Sequence
GCCCTACAACAACTAAGAAAATGGCTATCATGCGGAAGCTGGTGAGAAGAACAGCATCGGGACAAGGGAAGGAAGAACAAAGACAAAGAAAACAAAAGAAAGCAATTGAAAACAAAACAAAACAATTTTCATTCCTTCTCTTATCATTCCTTTTCTTTTCTTTTCTCTCATTCAACGCACTCCATCGTATCCGTATTCCTCTTATTTTTTCTCTTTCTCTATATCCATTTCTTTCTCTCTAGGTGTGTCCTCTCTCTCTCTTCAATTTCTCTACTCCGCATTCCAACGCATCCTTCCCCCAACCTCCCATTTCCTCCTTACGGCCCGATAGCGATCGTCTTTCCCTCGCTATCACTCGCTACCGGCCCCTCCTCTGCACCGTAACCTCCTACGTATTTACCATATCATAAAGTTTTTTCCGACGCTTATCGCTGACCCCCTGTCGCCCTCCTATTGGCTTCCGGATTATCTTCTTGTCCATAAGGTGATCCATGCTTCCTGAAGATTCCCGAAATGTGTCCACTTTGGCGGGGAATCATTCCATCCACTTCTTTCTCTCTCGCTTTCCTCATTCGGCGCTCCCCTTCCGCGTCTCATTGGTCTTCCGCTCCGTTTTTGCTTTGCCGATGTTACTTGGGGAGAGGTGCGATAATCCTTTCGCAAAAACTCGGTTTGACGCCTCCCATGGTAAATATAGTGGGTGGTGGACAGGTGCCTTCGCTTTTCTTTAAGCAAGAGAATT
TEF promoter — — 8704..9048 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
lac promoter — — 11511..11542 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 5..27 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
lac operator — — 11487..11504 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
rep_origin 2
Label Gene Product Position Strand
ori — — 314..903 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
ARS1 — — 7849..8631 Fwd
DNA Sequence
TCTATAATTATAGCTAAAAATTGTATTTTAATTTGTATTTTTTGTAATTTTATTTCTCATTGTTTTACTTAAAATGAATGCGAATTAGAGAAAACTTCAACGAAATGTCAAAATAAGCTCAGCAAAATATACAATTTTAGGGAAAGCGATCAGCAACTTATTCTCCGTTGTAGATTCTTTTTCTTCATAAATTCCAACAATGTAACTGCCAATTGATTTTTCTCTAGATGCTAATAAATTGAAACTAATGAATATAATAACACAGGTTTTGATAAATTGTAAACTCTTACTCAAAAAAAGAATGTTAAAACTAATTTAACCTTTTTATAAAGGCTCATGGCGTTAAAGAGGATAGTAAAAATTCTACAATTTTTTAATTTAATGTCACAATTGTCTTGCGAATATAATTCTATTAATTTTCGCTAACTGCTGTTTTAGTACTATTACTTTCAATATGCCTTATATGCAACTTTAATTCATGAAGAATGCAATATTTTTGCAATCTTTCTTGTACTGAGCGCTTTTTATCATTTAGTACTCATTATTTAATTTTTCAGTAAAGGGCAATAAATAAATTTGTAGAAGATGCAATGTAATCTCCTCTATCCTTTTGCTCATATGTTTATGAGTATACCTAGTCTAGAAAGGCTTGTATTTAAAATATTATTCAATAAAAATTCACAATTTTTACGACATGTGCTACCATTCACTTAACTTCCTGATTATAAAATTGGTTCGTTTATACTAATTACTTAAGTACCTTTAACTAAACAAAATGCCTA
terminator 5
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 6987..7235 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
TEF terminator — — 9626..9824 Fwd
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG
rrnB T1 terminator Escherichia coli rrnB — 11210..11282 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator — — 11297..11325 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
SUP4 terminator — — 11423..11443 Fwd
DNA Sequence
TTTTTTTGTTTTTTATGTCT