pRha-ABE8e-NRCH

165417 Synthetic Bacterial Expression

Plasmid Information

Purpose

For bacterial purification of ABE8e-NRCH protein

Depositor

David Liu

Insert

ABE8e-NRCH

Tags

8xHIS and BP-NLS

Promoter

pRhaBAD

Available Since

June 3, 2021

Availability

Available

Sequence Information

Length

7,095 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
SV40 NLS	—	nuclear localization signal of SV40 (si…	2723..2744	Fwd
DNA Sequence CCGAAAAAGAAACGCAAGGTG Protein Sequence PKKKRKV
KanR	aph(3')-Ia	aminoglycoside phosphotransferase	3843..4653	Rev
DNA Sequence ATGAGCAATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAATTTCATTTGATGCTCGATGAGTTTTTCTAA Protein Sequence MSNIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
8xHis	—	8xHis affinity tag	5000..5024	Fwd
DNA Sequence CACCATCACCATCATCATCATCAC Protein Sequence HHHHHHHH
SV40 NLS	—	nuclear localization signal of SV40 (si…	5057..5078	Fwd
DNA Sequence CCGAAAAAAAAGCGTAAAGTT Protein Sequence PKKKRKV

promoter 2

Label	Gene	Product	Position	Strand
cat promoter	—	—	4653..4744	Rev
DNA Sequence TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
rhaB promoter	—	—	4847..4966	Fwd
DNA Sequence CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA

rep_origin 1

Label	Gene	Product	Position	Strand
p15A ori	—	—	2982..3528	Fwd
DNA Sequence TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA

terminator 4

Label	Gene	Product	Position	Strand
T7 terminator	—	—	2778..2826	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T3Te terminator	—	—	2923..2953	Fwd
DNA Sequence GGCTCACCTTCACGGGTGGGCCTTTCTTCG
T7Te terminator	—	—	3792..3820	Rev
DNA Sequence GGCTCACCTTCGGGTGGGCCTTTCTGCG
tonB terminator	—	—	4752..4783	Rev
DNA Sequence AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC