pFA-MNase-SAT1

162465 Synthetic Yeast Expression

Plasmid Information

Purpose
pFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNase
Depositor
Adnane Sellam
Article
Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006
Insert
3FLAG-Mnase
Available Since
Jan. 5, 2021
Availability
Available

Sequence Information

Length
4,889 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
3xFLAG — three tandem FLAG(R) epitope tags, foll… 163..229 Fwd
DNA Sequence
GATTATAAGGACCATGACGGAGATTACAAAGATCACGACATTGACTACAAAGATGACGACGATAAA
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
MNase — micrococcal nuclease from Staphylococcu… 235..682 Fwd
DNA Sequence
GCCACATCTACCAAGAAACTTCATAAGGAACCAGCTACATTGATTAAGGCAATAGATGGGGACACCGTAAAACTTATGTACAAAGGGCAGCCAATGACCTTCAGATTGTTGTTGGTTGACACTCCTGAAACAAAACACCCAAAGAAAGGAGTGGAAAAATATGGTCCTGAGGCTTCCGCTTTCACAAAGAAAATGGTAGAAAACGCAAAGAAGATCGAAGTCGAGTTCGACAAAGGTCAACGTACCGATAAATATGGAAGAGGACTTGCATACATTTACGCCGATGGAAAAATGGTGAATGAAGCATTGGTGAGACAAGGGTTAGCAAAAGTTGCATATGTCTACAAGCCAAATAACACACACGAGCAGCATTTACGTAAATCAGAGGCACAGGCAAAGAAAGAGAAATTAAACATTTGGAGTGAGGATAATGCTGATTCAGGGCAA
Protein Sequence
ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ
AmpR bla beta-lactamase 3662..4523 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 3
Label Gene Product Position Strand
SP6 promoter — — 84..103 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA
T7 promoter — — 2627..2646 Rev
DNA Sequence
TAATACGACTCACTATAGG
AmpR promoter bla — 4523..4628 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 2903..3492 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA