pT-43

16232 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Yoshiko Kikuchi
Article
Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415
Insert
SMT3 (SMT3 Budding Yeast)
Tags
His
Available Since
Nov. 30, 2007
Availability
Available

Sequence Information

Length
761 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 171..189 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
SUMO S. cerevisiae SMT3 (truncated) cleavable ubiquitin-like protein tag 195..489 Fwd
DNA Sequence
ATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGT
Protein Sequence
MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
promoter 1
Label Gene Product Position Strand
T5 promoter 54..99 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator 74..91 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator 106..123 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator 526..621 Fwd
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT