pT-35

16092 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Yoshiko Kikuchi
Article
Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-
Insert
SMT3 (SMT3 Budding Yeast)
Tags
GST
Available Since
Nov. 30, 2007
Availability
Available

Sequence Information

Length
765 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 5..23 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
SUMO S. cerevisiae SMT3 (truncated) cleavable ubiquitin-like protein tag 23..317 Fwd
DNA Sequence
ATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGT
Protein Sequence
MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
promoter 1
Label Gene Product Position Strand
AmpR promoter bla 649..754 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT