pDEST17-UbE2T — PlasmidDB

Plasmid Information

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Length

829 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	58..76	Fwd
DNA Sequence CATCACCATCACCATCAC Protein Sequence HHHHHH

protein_bind 2

Label	Gene	Product	Position	Strand
attB1	mutant version of attB	—	85..110	Fwd
DNA Sequence ACAAGTTTGTACAAAAAAGTTGGGA
attB2	mutant version of attB	—	705..730	Rev
DNA Sequence ACCACTTTGTACAAGAAAGTTGGGT