pWM_12x601_193 NRL + TetO

Plasmid Information

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Available Since

March 17, 2022

Availability

Available

Length

4,718 bp

Topology

circular

Molecule Type

other DNA

promoter 3

Label	Gene	Product	Position	Strand
AmpR promoter	bla	—	2370..2462	Fwd
DNA Sequence TTTGTTTATTTTTCTAAATACATTCAAATAGATATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter	—	—	4061..4092	Fwd
DNA Sequence TTTACACTTTATGCTGCCGGCTCGTATGTTG
T7 promoter	—	—	4312..4331	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 3

Label	Gene	Product	Position	Strand
tet operator	tetO	—	801..820	Fwd
DNA Sequence TCTCTATCACTGATAGGGA
CAP binding site	—	—	4025..4047	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT
lac operator	—	—	4099..4116	Fwd
DNA Sequence TTGTGAGCGGATAACAA

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	3397..3985	Fwd
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTATGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGGATATCGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGTTCCGATATCGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA