pWM_12x601_30bpLinker

Plasmid Information

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Available Since

March 17, 2022

Availability

Available

Sequence Information

Length

4,155 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

promoter 2

Label	Gene	Product	Position	Strand
lac promoter	—	—	0..4155	Fwd
DNA Sequence TTTACACTTTATGCTGCCGGCTCGTATGTTG
AmpR promoter	bla	—	2450..2542	Fwd
DNA Sequence TTTGTTTATTTTTCTAAATACATTCAAATAGATATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	24..41	Fwd
DNA Sequence TTGTGAGCGGATAACAA
CAP binding site	—	—	4105..4127	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	3477..4065	Fwd
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTATGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGGATATCGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGTTCCGATATCGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA