pWM_12x601_20bpLinker

157787 Synthetic Bacterial Expression

Plasmid Information

Purpose
EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
Depositor
Michael Rosen
Article
Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi
Insert
12x601_20bp linker
Available Since
March 17, 2022
Availability
Available

Sequence Information

Length
4,035 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
AmpR promoter bla — 321..413 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATAGATATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter — — 2726..2757 Rev
DNA Sequence
TTTACACTTTATGCTGCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 2702..2719 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 2771..2793 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 2833..3421 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTATGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGGATATCGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGTTCCGATATCGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA