pAH18

154397 Synthetic Bacterial Expression

Plasmid Information

Purpose
Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
Depositor
Silvia Cardona
Article
Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
Insert
Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
Promoter
NA
Available Since
Aug. 31, 2020
Availability
Available

Sequence Information

Length
5,431 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
traJ traJ oriT-recognizing protein 765..1137 Fwd
DNA Sequence
ATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGA
Protein Sequence
MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP
TpR dhfrIIb from plasmid R388 E. coli plasmid-associated dihydrofolat… 4161..4398 Fwd
DNA Sequence
ATGGGTCAAAGTAGCGATGAAGCCAACGCTCCCGTTGCAGGGCAGTTTGCGCTTCCCCTGAGTGCCACCTTTGGCTTAGGGGATCGCGTACGCAAGAAATCTGGTGCCGCTTGGCAGGGTCAAGTCGTCGGTTGGTATTGCACAAAACTCACTCCTGAAGGCTATGCGGTCGAGTCCGAATCCCACCCAGGCTCAGTGCAAATTTATCCTGTGGCTGCACTTGAACGTGTGGCCTAA
Protein Sequence
MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGWYCTKLTPEGYAVESESHPGSVQIYPVAALERVA
promoter 1
Label Gene Product Position Strand
Pc promoter intI1 (promoter lies within the coding sequence) — 3807..3836 Fwd
DNA Sequence
TTGACATAAGCCTGTTCGGTTCGTAAACT
protein_bind 1
Label Gene Product Position Strand
FRT — — 2678..2726 Rev
DNA Sequence
GAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori — — 1754..2143 Fwd
DNA Sequence
GATCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACA