pGBW-m4046531

148995 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
Depositor
Ginkgo Bioworks, Benjie Chen
Insert
SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
Tags
Cleavable thrombin;6xHIS
Mutation
wt
Promoter
pTEF1
Available Since
May 14, 2020
Availability
Available

Sequence Information

Length
3,051 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 2865..2883 Fwd
DNA Sequence
CTCGTGCCGAGAGGTTCT
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 2883..2901 Fwd
DNA Sequence
CACCATCATCACCATCAT
Protein Sequence
HHHHHH