pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE

148565 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial Expression of Hs4EHP_52-234opt-R103LE149L
Depositor
Elisa Izaurralde
Article
Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.
Insert
Hs4EHP_52-234opt-R103LE149L (EIF4E2 Human)
Available Since
March 16, 2023
Availability
Available

Sequence Information

Length
4,967 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 3052..3073 Fwd
DNA Sequence
GAAAACCTTTACTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 3100..3118 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 3
Label Gene Product Position Strand
T7 promoter — — 2400..2419 Fwd
DNA Sequence
TAATACGACTCACTATAGG
tet promoter — — 3434..3463 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
cat promoter — — 4645..4748 Fwd
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
protein_bind 1
Label Gene Product Position Strand
lac operator — — 2419..2444 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 3574..4120 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 3212..3260 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG