pnYC-CvH-Hs4EHP_1-234-W95A_AE

Plasmid Information

Purpose

Bacterial Expression of Hs4EHP_1-234-W95A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-W95A (EIF4E2 Human)

Available Since

March 16, 2023

Availability

Available

Length

5,120 bp

Topology

circular

Molecule Type

other DNA

CDS 2

Label	Gene	Product	Position	Strand
TEV site	—	tobacco etch virus (TEV) protease recog…	3205..3226	Fwd
DNA Sequence GAAAACCTTTACTTCCAGGGC Protein Sequence ENLYFQG
6xHis	—	6xHis affinity tag	3253..3271	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 3

Label	Gene	Product	Position	Strand
T7 promoter	—	—	2400..2419	Fwd
DNA Sequence TAATACGACTCACTATAGG
tet promoter	—	—	3587..3616	Rev
DNA Sequence TTGACAGCTTATCATCGATAAGCTTTAAT
cat promoter	—	—	4798..4901	Fwd
DNA Sequence TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	2419..2444	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC

rep_origin 1

Label	Gene	Product	Position	Strand
p15A ori	—	—	3727..4273	Fwd
DNA Sequence TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	3365..3413	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG