pnYC-NpH-HseIF4E_36-217_AA

148282 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial Expression of HseIF4E_36-217
Depositor
Elisa Izaurralde
Article
Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi
Insert
HseIF4E_36-217 (EIF4E Human)
Available Since
March 16, 2023
Availability
Available

Sequence Information

Length
3,320 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 850..868 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
HRV 3C site — recognition and cleavage site for human… 889..913 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 3
Label Gene Product Position Strand
T7 promoter — — 751..770 Fwd
DNA Sequence
TAATACGACTCACTATAGG
tet promoter — — 1787..1816 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
cat promoter — — 2998..3101 Fwd
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
protein_bind 1
Label Gene Product Position Strand
lac operator — — 770..795 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 1927..2473 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 1565..1613 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG