pQE9-His-p97(QQ)

14667 Mus musculus Bacterial Expression

Plasmid Information

Depositor
Graham Warren
Article
Ye et al Nature. 2001 Dec 6. 414(6864):652-6.
Insert
p97 (Vcp Mouse)
Tags
RGS6xHis
Mutation
E305/578Q
Available Since
April 13, 2007
Availability
Available

Sequence Information

Length
756 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 154..172 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T5 promoter — — 37..82 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 57..74 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 89..106 Fwd
DNA Sequence
TTGTGAGCGGATAACAA