pAV0751

133485 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus
Depositor
Sophie Martin
Article
Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.
Insert
pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
Available Since
Nov. 22, 2019
Availability
Available

Sequence Information

Length
8,079 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
S. pombe ura4 Schizosaccharomyces pombe ura4 orotidine 5'-phosphate decarboxylase, r… 1639..2434 Fwd
DNA Sequence
ATGGATGCTAGAGTATTTCAAAGCTATTCAGCTAGAGCTGAGGGGATGAAAAATCCCATTGCCAAGGAATTGTTGGCTTTGATGGAAGAAAAGCAAAGCAACTTGTCAGTCGCGGTCGATTTGACGAAGAAATCCGAAATCTTAGAATTGGTAGATAAAATTGGACCCTATGTCTGTGTTATCAAGACACATATTGACGTTGTCGAGGATTTCGACCAGGATATGGTAGAAAAACTGGTGGCCTTAGGTAAAAAGCATCGTTTTCTTATCTTTGAGGATCGCAAATTCGCAGACATTGGAAATACCGTCAAGCTACAATATGCATCTGGTGTGTACAAAATTGCTTCTTGGGCTCATATCACAAATTGCCATACAGTGCCAGGCGAGGGTATTATACAAGGCCTCAAAGAAGTTGGTTTACCTTTGGGACGTGGTCTCTTGCTTTTGGCTGAAATGTCTTCCAAAGGCTCTTTGGCTACTGGTTCCTACACAGAGAAAACCTTAGAATGGTTTGAGAAGCATACCGATTTTTGCTTTGGCTTTATAGCTGGTCGTCGATTTCCTAACCTTCAAAGCGACTACATAACTATGTCCCCTGGTATCGGCTTGGATGTTAAAGGAGACGGGCTGGGACAGCAATATCGTACTCCTGAAGAAGTGATTGTAAACTGCGGTAGCGATATCATCATTGTTGGTCGTGGAGTTTATGGAGCTGGTCGTAATCCTGTTGTCGAAGCCAAGAGATATAGAGAAGCTGGTTGGAAGGCATATCAGCAAAGACTTTCTCAGCATTAA
Protein Sequence
MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKSEILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSYTEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIVNCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH
AmpR bla beta-lactamase 7018..7879 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 5
Label Gene Product Position Strand
T7 promoter — — 3547..3566 Fwd
DNA Sequence
TAATACGACTCACTATAGG
nmt1 promoter — — 3607..4771 Fwd
DNA Sequence
TCGCCATAAAAGACAGAATAAGTCATCAGCGGTTGTTTCATTTCCTATATTTTTTTTTTATTTTTTTATTTTTTAATAAGGGAAAATTTAACGTCTAAGGATACAGAAGATTGTTAGCACATTAAAGTAATAAAGGCTTAAGTAGTAAGTGCCTTAGCATGTTATTGTATTTCAAAGGACATAATCTAAAATAATAACAATATCATTTCTCACAAGTTATTCAATTTTCTTTTTTTTTTCTAATAATATCAAGAATGTATTATTTGTTTGACATAAGTCAACTAATTTATTTAATATGCTGGATTAATCTTGCAGACATGTAAATTAACAAGTTTTAGTCAAATAACGTTGAAGTTTCAATGAACTCAAATAATTTCTCTTTTTTTTTATATAACCATATGTCTAATCTGATTTATATTTTCCGCAGGGATCAACTGAAGTTATGACATTTGGATTGGATCACTTATAACCTTGGTCGCCAAATAATACAAAAATCAGCGTTATAAAACAAAGAAGGTTTTTGTTAAGAAATTAATCCTCTTTCTTGATAAGAAAGTTGAACCGAAATTGCAGATACTGATATATGAAAATAATACCCACAATTTTGGGAATAGCGCAAGCCTCAATTTAAACAATAGGTGAGGACACATGATAATGACCTCAATGATTGTTAGAAGAAAAGAGCCTCATTACAAAATCGAAAAATGAATGGTTGGGTACAAGTTTCCAAAACATGGTAAAGTGGACTTTGCGTATGAGACGTAAATAGAAAAAAACACTTGTTATATGTTTTCTAGAATTATTGTTGTCTCTTTATGGTTGGATGATGCAAAATAGTAATTTCGGTTAGTTGCTGTAAAACACCACGAGACAAATAGATATGGATATTTATTAAATCAGGAAAAACGTAACTCTCGGCTACTGGATGGTTCAGTCACCCAACGATTACTGGGGAGAGAAAACAGGGCAAAAGCAAAGCTTAAAGGAATCCGATTGTCATTCGGCAATGTGCAGCGAAACTAAAAACCGGATAATGGACCTGTTAATCGAAACATTGAAGATATATAAAGGAAGAGGAATCCTGGCATATCATCAATTGAATAAGTTGAATTAATTATTTCAATCTCATTCTCACTTTCTGACTTATAGTCGCTTTGTTAAATC
T3 promoter — — 5818..5837 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 5905..5936 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla — 7879..7984 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator — — 5881..5898 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 5950..5972 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 2925..3381 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori — — 6259..6848 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 5544..5794 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC